The Montclair University team utilizes the Advion CMS in their undergraduate lab. Essential in the research-focused curriculum, the team was able to purchase the cost effective, small-footprint system to grow their lab capabilities. They later added an APCI module for their growing needs.
A Medicinal Chemist’s View on using the Advion CMS
Explore how the expression CMS has been used by the Drug Discovery Unit at the University of Dundee. Learn how the medical chemistry team has integrated the instrument in to their everyday workflow. The team required a system with a small footprint that can be moved between labs, but was still capable of integrating with systems in their well-equipped laboratories. The expression CMS fit the needs of the organization and increased efficiencies in the lab.
Determination of emerging nitrogenous economic adulterants in milk proteins by high-performance liquid chromatography/compact mass spectrometry
J. Draher, S. Ehling, N. Cellar, T. Reddy, J. Henion, N. Sousou Rapid Commun. Mass Spectrom. 2016, 30, 1265–1272
Milk-derived ingredients are widely used around the world in the manufacturing of nutritional products. They are prone to economically motivated adulteration with nitrogenous compounds such as melamine and its analogs in order to increase the nitrogen content of these ingredients. The need to rapidly screen milk-derived ingredients to detect adulteration is of paramount public health concern. A liquid chromatography/mass spectrometry (LC/MS)-based method using a single quadrupole mass spectrometer has been developed for the rapid frontline analysis of six nitrogenous protein adulterants, i.e. melamine, ammeline, ammelide, amidinourea, cyromazine and cyanuric acid, in three key milk-derived ingredients, i.e. whole milk powder, nonfat milk powder and whey protein concentrate.
Protein Analysis via LC/CMS with Automated Charge State Deconvolution
The Advion deconvolution algorithm employs a combination of predictive charge envelope generation, overlap calculation with actual MS data acquired utilizing a maximum entropy goodness of fit approach followed by artifact removal through determination of peak significance. This novel approach allows for a rapid deconvolution over a wide mass range in seconds. It also allows a neutral mass output where the absolute signal intensity is directly correlated to the MS raw data signal intensity and analyte concentration in solution.
Polish Academy of Sciences, Warsaw, PL
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: The group has extensive experience in host-guest chemistry. We are seeking novel supramolecular platforms/systems, in particular macrocyclic systems, which might find applications in the recognition of ionic and neutral guest molecules. The research consists of two main parts: synthesis of novel receptors and analysis of their supramolecular assemblies. The formation of supermolecules is monitored and analyzed by NMR, UV-vis and fluorescence spectrometry. X-ray analysis is used to determine structure of receptors and their complexes. We also work with dynamic combinatorial libraries (DCL) and occasionally synthesize isotopically labeled compounds.
Q: WHAT WAS YOUR PREVIOUS WORK FLOW OR CHALLENGES?
A: In the synthesis of molecules of complex structures we need fast and reliable feedback about the success of each synthetic step. For example, macrocyclisation reactions sometimes give very complex mixtures. In order to follow the reaction course and facilitate purification, we need a fast tool for the unambiguous confirmation in which TLC spot is the desired product. Also, distinguishing between [1+1] and [2+2] macrocycles is not possible by simple NMR measurements and for new dynamic combinatorial libraries any HPLC characterization method required validation by an LC-MS in the service laboratory which takes time.
Q: WHY DID YOU INCORPORATE THE EXPRESSION® CMS INTO YOUR LABORATORY?
A: We needed a versatile tool to speed up our synthesis processes and facilitate purification protocols. The expression® CMS is mainly applied with Plate Express™ for fast compound identification from TLC plates. Now the choice of synthetic protocol can be evaluated within minutes. The product unambiguously spotted on the TLC plate is much easier to isolate. In addition, whenever needed we connect CMS to our HPLC system to analyze complex mixtures and to validate the analytical protocols. Isotope composition of labeled compounds is also easy to follow using this system.
Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE EXPRESSION® CMS?
A: We would recommend it to all groups dealing with synthesis of small molecules. The instant feedback about the reaction progress and aid in isolation of the product saves a lot of time. With the expression® CMS, the group is more independent, using the system as a stand-alone unit, coupled to the TLC plate reader or LC-system when needed.
University of Hull, Chemistry Department
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: My group are actively researching the application of mass spectrometry to a range of problems in both chemical and biomedical science. In particular we are involved in the application of mass spectrometry based proteomics for label free quantitation of proteins from human tissue samples and also in the identification of trace analytes from environmental samples. We are also investigating the synthesis of novel co-polymeric monolithic phases for sample extraction and separation.
Q: WHAT WAS YOUR PREVIOUS WORK FLOW OR CHALLENGES?
A: The Chemistry Department at Hull have long recognised the need for practical training on a wide range of chemical instrumentation. In particular we recognise the requirement from many companies in the pharmaceutical and fine chemical sectors for practical training in GC/MS and LC/MS. The Advion expressionL compact mass spectromater (CMS) was purchased to provide such training at an undergraduate level and has been incorporated into the range of instrumental analysis practicals for final year students. The expressionL CMS fits tidily on the bench-top next to the HPLC system and gives students the chance experience at first hand the use of LCMS as an analytical technique. The ease of use and robust nature of the system means that the expressionL CMS lends itself to the undergraduate laboratory where users will not be experts in mass spectrometry at this point in their careers.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: We use the expressionL CMS to give undergraduate students practical experience of using LC/MS to prepare and analyse a ‘mock’ urine sample. The sample has to be prepared by adding the internal standard and extracting the analytes using Solid Phase Extraction (SPE) to obtain a sample suitable for LC/MS analysis. The aim is to identify and quantify the compounds found in the sample by reference to the internal standard added. The HPLC separation is a rapid 10 min gradient elution and the ability to incorporate the data from the UV detector into the MS data provides an additional benefit. Purchasing the expression L CMS has allowed us to provide practical training in LC/MS for our undergraduate students at a time when prospective employers are actively seeking such skills.
Compact Mass Spectrometry (CMS) for Reaction Monitoring and Quality Control of Modern Solid Phase Peptide Synthesis (SPPS)
A recent development in mass spectrometry is the design of smaller sized MS systems with full analytical capability (compact mass spectrometry, CMS). CMS systems with fragmentation analysis are very cost eff ective analysis solutions and ideally suited for reaction and quality control in SPPS including detailed product characterization3. Here, we evaluate the utility of a system comprised of an automated peptide synthesizer, Prelude X
(Protein Technologies Inc.) and the LC/CMS analysis system expression-L (Advion Inc.) based on two peptides selected for their known synthetic challenge.
LC/MS Screening For EDTA In Fresh Blood Swabs Using the Advion Interchim Scientific expression® CMS System
This application note suggests that a simple LC/MS screen of a relatively fresh sample of human whole blood collected from a lavender capped potassium EDTA anticoagulant blood sample applied to a cotton swab can be analyzed by full-scan positive ion or negative ion LC/MS using a compact single quadrupole mass spectrometer (expression® CMS). A presumptive positive determination from such a test would benefit from confirmation by LC/MS/MS or other definitive confirmative analytical technique.
Qualitative Analysis of Commonly Abused Drugs by LC/MS on the expression® CMS
D. Tranchemontagne, N. Sousou, S. J. Prosser, J.D. Henion – Advion, Inc. & S. Orlowicz, S. Sadjadi, L. Snow – PhenoLogix
The Advion Interchim Scientific expression® Compact Mass Spectrometer (CMS) coupled with Liquid Chromatography (LC) was used as a qualitative screening tool. This application note will demonstrate the screening of various drugs, including amphetamines, benzodiazepines, antidepressants, opiates and opioid drugs as well as their common metabolites. It will show that the expression® CMS can easily be implemented in a doctor’s office to provide fast and easy drug screening.
Online quantitative mass spectrometry for the rapid adaptive optimisation of automated flow reactors
N. Holmes, G.R. Akien, R.J.D. Savage, C. Stanetty, I.R. Baxendale, A.J. Blacker, B.A. Taylor, R.L. Woodward, R.E. Meadows, and R.A. Bourne
An automated continuous reactor for the synthesis of organic compounds, which uses online mass spectrometry (MS) for reaction monitoring and product quantification, is presented. Quantitative and rapid MS monitoring was developed and calibrated using HPLC. The amidation of methyl nicotinate with aqueous MeNH2 was optimised using design of experiments and a self-optimisation algorithm approach to produce >93% yield.
