During this webinar you will learn more about:
- A new type of mass spectrum: the Delta Spectrum (ΔS). The patented ΔS is able to look beyond chemical noise and automatically detect even the smallest peaks without knowing the m/z.
- How to easily find adducts, dimers, fragments, side-reactions and other unexpected compounds at the click of a button, in real-time or with post-processing.
- How to use Peak Express software to highlight minor components in complex mixtures and dirty matrices, and can give you the ability to control mass-directed purification without providing the compound mass.
- How to easily acquire XIC-quality data while scanning the entire mass range, allowing Peak Express™ to tell you the m/z.This webinar was featured by Lab Manager’s Tech Trends webinar series, “Innovations in Mass Spectrometry.”
In this application note, a simple and cost-effective SIM LC/MS analytical approach employing the Advion expression Compact Mass Spectrometer (CMS) is described for the quantitation of 25-hydroxyvitamin D2 and D3 in human serum.
Reaction monitoring is a key aspect in a range of chemistry environments from chemical synthesis to drug discovery to understanding natural products to protein synthesis. Understanding the optimal time to quench a reaction for maximum yield, as well as monitoring a reaction in real-time is vital to many medicinal and synthetic organic chemists. Advion Interchim Scientific’s expression® CMS was developed with the chemist in mind to optimize their workflow directly at the bench. It is an easy-to-use and maintain single quadrupole detector that integrates with the industry’s broadest range of innovative sampling techniques from direct probe analysis to ultra-high performance liquid chromatography. Users can rapidly switch between the many different sampling techniques required throughout the chemist’s workflow.
University of Naples Federico II, Hospital Cardarelli, Coop. Samnium Medica, UCCP
Abstract
Atherosclerotic cardiovascular diseases are preferential targets of healthy diet and preventive medicine partially through strategies to improve lipid profile and counteract oxidative metabolites. Ninety individuals with cardiovascular disease (CVD) risk factors were randomized (1:1:1 ratio) to three arms, according to a four-week run-in, eight-week intervention, and four-week follow up study, testing the effects of a lactofermented Annurca apple puree (lfAAP), compared to unfermented apple puree (AAP) or probiotic alone (LAB) on plasma lipid profile and trimethylamine-N-oxide (TMAO) levels. By comparing the treatments, data indicated for the subjects tested with lfAAP a higher variation of the following serum parameters, in respect to the other treatment groups: high density lipoprotein cholesterol (HDL-C), +61.8% (p = 0.0095); and TMAO levels, −63.1% (p = 0.0042). The present study would suggest lfAAP as an effective functional food for beneficial control of plasma HDL-C and TMAO levels.
HPLC-DAD/ESI-MS analysis using the Advion expression® Compact Mass Spectrometer (CMS) was used to quantify TMAO in blood samples.
Federico II University Medical School of Naples, University of Naples “Federico II”, Institute for Hospitalization and Healthcare SDN
Abstract
Objective. Sex exerts an important influence on food preferences. The Mediterranean diet (MD) is based on the common dietary characteristics and lifestyle behaviors of the Mediterranean countries. Trimethylamine N-oxide (TMAO), a marker of gut dysbiosis linked to the development of atherosclerosis and cardiovascular risk, is mainly dependent on dietary pattern and gut microbiota metabolism. The aim of this study was to evaluate the association between levels of TMAO and adherence to the MD as a function of sex.
Bring mass spectrometry into your classroom for remote or on-site learning with a training course for the expression® Compact Mass Spectrometer (CMS).
Advion offers an affordable MS teaching package that includes:
- A compact, easy-to-use benchtop mass spectrometer for both students and researchers
- Recorded lectures and video lab content
- Lecture slides to share or adapt to your own teaching style
PLUS: Easy, student-friendly software. Select the workflow, the type of compounds, and students are on their way with just a few clicks of the mouse.
A research-grade mass spec, the expression® CMS is ideal for teaching because of its small size, ease of use, and one-click software. When class is not in session, the system is ready for research, making this a versatile choice for university labs seeking state of the art equipment paired with streamlined teaching modules.
Integrated Solutions for Food Authenticity, Characterization and Safety with the expression® Compact Mass Spectrometer, AVANT® (U)HPLC and SOLATION® ICP-MS
Your integrated laboratory solution for comprehensive testing of food, ingredients and natural products. Advion offers a full suite of analytical instrumentation for characterization, screening and purity determination, including:
- expression® CMS – The expression® Compact Mass Spectrometer (CMS) offers the industry’s widest range of sample introduction systems, providing rapid answers with little or no sample prep in < 30 seconds.
- SOLATION® ICP-MS – Provides fast, simple, multi-element analysis of toxic metals, and essential elements and minerals in a wide array of samples, including water, soil, plant material, food and raw ingredients
- AVANT® HPLC & UHPLC – Advion’s range of modular high performance, liquid chromatography systems can be used standalone with UV and UV/Vis detector options, or with the expression® CMS to provide seamlessly integrated LC/CMS under the full control of Advion’s simple, intuitive software suite.
- PLATE EXPRESS® TLC PLATE READER – Plate Express® provides a simple, automated means of obtaining mass spectra directly from TLC plates, combined with Advion’s expression® CMS creating a technique known as TLC/ CMS. See results in < 30 seconds at the push of a button.
Integrated Solutions for Potency and Safety with the expression® Compact Mass Spectrometer and SOLATION® ICP-MS
Outfit your lab with Advion Interchim Scientific’s line of custom cannabis solutions, including the expression® Compact Mass Spectrometer (CMS), the SOLATION® ICP-MS, and a customizable (U)HPLC solution.
Your Complete Lab Solution: Advion Interchim Scientific provides integrated laboratory solutions for comprehensive cannabis testing, including potency testing and cannabinoid levels as well as pesticide identification and quantitation of heavy metals.
Providing simple, high-performance, LC/CMS with the expression® CMS
Modular, stackable design, with many options provides custom solutions for both HPLC and UHPLC needs. From the simplest manual injection HPLC to a fully automated, streamlined UHPLC system and everything in-between, the Avant series can be configured to fit your analytical requirements and your budget.
The AVANT® (U)HPLC series is the latest in Advion’s innovative line of sample inlet devices for the expression® CMS which include:
- Plate Express® for automated mass assays from TLC plates
- The ASAP® direct analysis probe for solid, liquid and even air sensitive samples using iASAP
- vAPCI for analysis of volatile organic compounds (VOCs) from head spaces, breath, and other gas samples.
- Flow chemistry interfaces
All these techniques can be simply, simultaneously, interfaced with the CMS so that the chemist can move quickly and effortlessly between workflows.
Introduction
There are many separation and detection methods that are available for the determination of saccharides. These include GC-MS with derivatization, HPLC-UV with derivatization, HPAEC-PAD (High-performance anion-exchange chromatography coupled with pulsed amperometric detection), and LC-MS. The availability of a sensitive and accurate method is still a challenge. For an LC-MS method, given the high polarity, hydrophilicity and low proton affinity of these compounds, it can be difficult to ionize them directly by electrospray by ESI or APCI. The chemical diversity and numerous isomers of carbohydrates further complicates their analysis with LC-MS.
In this Application Note, post-column chloride attachment is used for the analysis of samples containing carbohydrates using the Advion expression® Compact Mass spectrometer (CMS). The 35Cl and 37Cl isotopes also provide confirmation for the formation of the chloride adducts in negative ion APCI mode. Four carbohydrates (Fructose, Glucose, Sucrose and Lactose) are used for method verification.
