Breaking Through Bottlenecks in Organic Synthesis with a Streamlined Purification Workflow

Successfully completing an organic synthesis involves three typically lengthy, but crucial steps: reaction monitoring, compound identification, and purification. These steps can create bottlenecks in the synthesis, unless a purpose-built, streamlined, and reliable workflow is on hand.
This whitepaper delves into some of the challenges faced by synthetic organic chemists as they navigate compound identification and purification struggles. It also describes how using a workflow that couples thin layer chromatography (TLC) and mass spectrometry (MS) to flash purification can help increase the efficiency of the entire process.

Key Objectives：

  • Understand how a workflow that combines thin-layer chromatography, mass spectrometry, and flash purification can increase the speed and efficiency of an entire organic synthesis process.
  • Learn about prep-free techniques that have become the new standard: scraping TLC spots, developing flash methods and performing long liquid chromatography/MS runs are not necessary with modern instrumentation that is specifically developed to take the prep-work and guess-work out of the purification process.
  • Become informed about a cost-effective, user-friendly benchtop workflow solution for organic compound identification and purification that can save time and money for any lab.

Simplifying the Flash Purification Process Leveraging TLC and Mass Spectrometry

The compound synthesis, purification and confirmation processes often present challenges. From ensuring successful synthesis of your product, setting up a flash purification method, and finally confirming ID of fractions, the lengthy work flow can be time consuming and repetitive.

This webinar will provide a foundation for flash purification processes, and will showcase a novel work flow concept that breaks down the process in to easy steps for optimal success. We will offer tips to optimize each essential segment of the workflow, and share seamless steps to quickly and easily drive the process from reaction monitoring to flash purification and fraction ID.

Presented by Dr. Daniel Eikel, Director of Customer Service and Product Applications, Advion.

This webinar was hosted by C&EN and recorded 8/13/20.

Intelligent Flash Purification: TLC Guided Purification and Mass Spectrometry

The combination of the Advion Interchim Scientific expression® CMS and puriFlash® flash chromatography technologies speed up the synthesis, purification and characterization of a compound.

Using the Advion Interchim Scientific expression® Compact Mass Spectrometer, the Plate Express™ TLC Plate reader, the ASAP® Atmospheric Solids Analysis Probe, and the puriFlash® XS520 flash purification system, it is now possible to execute a fast, user-friendly workflow to run TLC, harness the power of your cell phone to develop a flash method, and finally utilize a simple dipping probe to confirm fractions in seconds.

With easy sampling techniques, intuitive software, and advanced detection technology, you can easily and confidently synthesize, purify and characterize your compound, in a flash.

Intelligent Flash Purification using TLC and Mass Spectrometry

The compound synthesis, purification and confirmation process often presents challenges throughout. From ensuring successful synthesis of your product, setting up a flash purification method, and finally confirming ID of fractions, the lengthy work flow can be time consuming and repetitive.

In this application note, we demonstrate how the combination of Advion Interchim Scientific technologies speed up the synthesis, purification and characterization of a compound.

This series features the Advion Interchim Scientific expression® Compact Mass Spectrometer (CMS), the Plate Express™ TLC Plate Reader, the Atmospheric Solids Analysis Probe (ASAP®), and the puriFlash® XS520 flash purification system.

The Essential Systems & Consumables for Purification, Evaporation & Mass Spectrometry

The Advion Interchim Scientific suite of systems and consumables allows users to harness the power of mass spectrometry, flash chromatography, prep LC and more. Simple and robust, download our brochure to learn more about:

  • The Advion Interchim Scientific expression® compact mass spectrometer (CMS): A fast and easy analytical tool for the organic chemist. Ideal for fast reaction monitoring, the expression® CMS features a single quadrupole that can adapt to multiple ionization sources in seconds, including both ESI and APCI. The expression® CMS offers a variety of novel sampling techniques, including fast assay methods for liquids, solids, gases, and even air-sensitive compounds.
  • Direct mass analysis of TLC plates in 30 seconds at the push of a button with Plate Express™
  • One-touch analysis of solids and liquid samples with the ASAP® probe
  • LC/CMS
  • puriFlash® ultra performance flash purification: Ideal for method development and purification of rare and high added value compounds, the Interchim family of puriFlash® systems offer users a wide range of throughput options and the highest recovery rates at >95%.
  • Mass-Guided Purification: the Interchim puriFlash® + Advion CMS offers the ideal solution for Flash-MS, and can provide fraction identification in <30 seconds.

Fill out the form to download the full Advion Interchim Scientific brochure now.

expression® CMS: Mass Spectrometers Designed for Chemists

With decades of mass spectrometry and chemistry expertise, Advion Interchim Scientific has produced a family of compact mass spectrometers designed for the chemist. The affordability, small size and ease of use make them ideal for use directly at the chemist’s bench, giving immediate answers and informed decisions instead of waiting.

The Advion Interchim Scientific expression® compact mass spectrometer (CMS) is a fast and easy analytical tool for the organic chemist. Ideal for fast reaction monitoring, the expression® CMS features a single quadrupole that can adapt to multiple ionization sources in seconds, including both ESI and APCI. The mass spectrometer works in a variety of applications, including food and beverage, pharmaceutical, biomedical, peptides and proteins, drug discovery and more. The expression® CMS offers a variety of novel sampling techniques, including:

  • Direct mass analysis of TLC plates in 30 seconds at the push of a button with Plate Express™
  • One-touch analysis of solids and liquid samples with the ASAP® probe
  • LC/CMS
  • Many more options and configurations to suit your needs

Learn more about the different sample techniques available with the expression® CMS, including fast assay methods for liquids, solids, gases, and even air-sensitive compounds.

Fill out the form to download the full expression® CMS brochure now.

Mass Directed Fraction Collection

The Advion expression Compact Mass Spectrometer (CMS) was coupled to a Normal Phase Flash Chromatography system for mass directed fraction collection. Typically a UV detector is used to monitor compounds eluting from Normal Phase columns. A limitation of the UV detector is that it relies on compounds having a chromophore in the chemical structure, hence an analyte of interest may not be detectable if it lacks a suitable UV chromophore or coelutes with another compound. In addition, solvent systems can interfere with and hinder UV absorbance. The Advion expression CMS provides the ultimate in selectivity, unambiguously identifying residual starting materials, desired product ions, side reactants and impurities by their molecular weight despite co-eluting compounds and solvent peaks. Mass directed fraction collection greatly speeds up the chemist’s work flow with confidence in the content and purity of the collected fractions by eliminating off-line TLCs, workup and core lab LC/MS assays.