Liquid Extraction Surface Analysis for Native Mass Spectrometry: Protein Complexes and Ligand Binding

Published by the International Journal of Mass Spectrometry

Native liquid extraction surface analysis (LESA) mass spectrometry enables the direct sampling of protein complexes from a solid surface. We have previously demonstrated native LESA mass spectrometry of holomyoglobin (~17 kDa) from glass slides and tetrameric haemoglobin (~64 kDa) from dried blood spots and thin tissue sections. Here, we further explore the capabilities of this
emerging technique by investigating a range of proteins which exist in various oligomeric states in vivo.

Victor A.Mikhailov, Rian L.Griffiths, Helen J.Cooper,
Liquid Extraction Surface Analysis for Native Mass Spectrometry: Protein
Complexes and Ligand Binding, International Journal of Mass Spectrometry
http://dx.doi.org/10.1016/j.ijms.2016.09.011