LESA – Liquid Extraction Surface Analysis

More analytes and higher sensitivity

Developed in collaboration with Oak Ridge National Laboratory*, the liquid extraction surface analysis (LESA) capability of the TriVersa Nanomate® enables simple, direct ESI mass spectrometric analysis from a variety of surfaces.

Using the TriVersa NanoMate® for Rapid Analyte Identification Based on Static nESI-MS/MS (Infusion MS/MS) Employing Polarity Switching Experiments

Automated static nano-ESI/MS/MS (Infusion MS/MS) is an easy-to-use and high-throughput approach to analyte identification. It is particularly useful in the field of shotgun lipidomics, which itself has great potential for biomarker discovery and gained some significant attention in the last couple of years (Schuhmann et al. 2012; Jung et al. 2011; Han et al. 2011).

In order to simplify shotgun lipidomic approaches even further, the TriVersa NanoMate can now change nano-ESI spray polarity within the same infusion experiment.

Using the TriVersa NanoMate® for Rapid Analyte Identification Based on Static nESI-MS/MS (Infusion MS/MS)

In many cases of routine protein identification, processing times become the major bottleneck at the core facility. Although a nano-LC/MS/MS approach is recognized as a general strategy for protein identification, the typical 30-120 min run-time prohibits fast turnaround times. The TriVersa NanoMate offers a more rapid approach for the infusion of the protein digest, with only a 2-5 min run-time per sample.