Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: Almost exclusively high throughput lipid profiling of samples from large epidemiological studies, where we study gene-lifestyle interactions. The method works with plasma samples as well as dried blood spots. The method is also applied to small-scale studies of specific disease groups, dietary interventions and model systems such as yeast. The TriVersa NanoMate is also used to study the lipid composition of tissues analyzed by LESA.
Q: WHY DID YOU INCORPORATE THE TriVersaNanoMate INTO YOUR LABORATORY?
A: The TriVersa NanoMate is essential to efficiently analyze large-scale studies. It offers a really robust method for high throughput studies with minimal carryover.
Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE TriVersa NanoMate?
A: I recommend the TriVersa NanoMate to laboratories with a large number of samples requiring a robust and reliable delivery system. The TriVersa NanoMate eliminates typical nanoelectrospray ionization challenges.
Q: DO YOU HAVE ANY PUBLICATIONS OR PRESENTATIONS USING THE TriVersa NanoMate?
A: Koulman, A.; Prentice, P.; Wong, M.C.Y.; Matthews, L.; Bond, N.J.; Eiden, M.; Griffin, J.L.; Dunger, D.B., The development and validation of a fast and robust dried blood spot based lipid profiling method to study infant metabolism, Metabolomics 2014.
A novel surface analysis platform based on LESApluswith LC separation and ion mobility mass spectrometry includes an improved LESA resolution of 400 µm, 2D LC desalting and separation step as well as ion mobility gas phase separation of isobaric compounds to identify neuropeptides from rat pituitary gland tissue.
Learn more about Liquid Extraction Surface Analysis PLUS LC separation with the Advion TriVersa NanoMate featuring new LESAPlus technology.
ABSTRACT: Large-scale metabolic profiling requires the development of novel economical high-throughput analytical methods to facilitate characterization of systemic metabolic variation in population phenotypes. We report a fit-forpurpose direct infusion nanoelectrospray high-resolution mass spectrometry (DI-nESI-HRMS) method with time-of-flight detection for rapid targeted parallel analysis of over 40 urinary metabolites. The newly developed 2 min infusion method requires <10 μL of urine sample and generates high-resolution MS profiles in both positive and negative polarities, enabling further data mining and relative quantification of hundreds of metabolites. Here we present optimization of the DI-nESI-HRMS method in a detailed step-by-step guide and provide a workflow with rigorous quality assessment for large-scale studies.
From the Journal of Proteome Research, Published February 2017
The TriVersa NanoMate® LESA® is the latest in chip-based electrospray ionization technology from Advion. It combines the benefits of liquid chromatography, mass spectrometry, chip-based infusion, fraction collection and direct surface analysis into one integrated ion source platform. It allows scientists to obtain more information from complex samples than LC/MS alone.