University of Cambridge, NIHR BCR Metabolomics and Lipidomics Facility

Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?

A: Almost exclusively high throughput lipid profiling of samples from large epidemiological studies, where we study gene-lifestyle interactions. The method works with plasma samples as well as dried blood spots. The method is also applied to small-scale studies of specific disease groups, dietary interventions and model systems such as yeast. The TriVersa NanoMate is also used to study the lipid composition of tissues analyzed by LESA.

Q: WHY DID YOU INCORPORATE THE TriVersaNanoMate INTO YOUR LABORATORY?

A: The TriVersa NanoMate is essential to efficiently analyze large-scale studies. It offers a really robust method for high throughput studies with minimal carryover.

Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE TriVersa NanoMate?

A: I recommend the TriVersa NanoMate to laboratories with a large number of samples requiring a robust and reliable delivery system. The TriVersa NanoMate eliminates typical nanoelectrospray ionization challenges.

Q: DO YOU HAVE ANY PUBLICATIONS OR PRESENTATIONS USING THE TriVersa NanoMate?

A: Koulman, A.; Prentice, P.; Wong, M.C.Y.; Matthews, L.; Bond, N.J.; Eiden, M.; Griffin, J.L.; Dunger, D.B., The development and validation of a fast and robust dried blood spot based lipid profiling method to study infant metabolism, Metabolomics 2014.

Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS): Examples of a New Surface Probing Technique for Clinical and Pre-Clinical Applications

Executive Summary:

  • LESA and LESAplus liquid chromatography provide novel surface analysis tools for the spatial resolution of analytes from a wide variety of surfaces.
  • Whole body or organ sections in particular can be probed for small molecules, lipids and proteins with a resolution of 400 – 1000μm.
  • Commercial solution allows for automated processing of samples (Advion TriVersa NanoMate® LESA®).

Optimization and Application of Direct Infusion Nanoelectrospray HRMS Method for Large-Scale Urinary Metabolic Phenotyping in Molecular Epidemiology

ABSTRACT: Large-scale metabolic profiling requires the development of novel economical high-throughput analytical methods to facilitate characterization of systemic metabolic variation in population phenotypes. We report a fit-forpurpose direct infusion nanoelectrospray high-resolution mass spectrometry (DI-nESI-HRMS) method with time-of-flight detection for rapid targeted parallel analysis of over 40 urinary metabolites. The newly developed 2 min infusion method requires <10 μL of urine sample and generates high-resolution MS profiles in both positive and negative polarities, enabling further data mining and relative quantification of hundreds of metabolites. Here we present optimization of the DI-nESI-HRMS method in a detailed step-by-step guide and provide a workflow with rigorous quality assessment for large-scale studies.

From the Journal of Proteome Research, Published February 2017

TriVersa NanoMate® LESA® with ESI Chip® Technology

The TriVersa NanoMate® LESA® is the latest in chip-based electrospray ionization technology from Advion. It combines the benefits of liquid chromatography, mass spectrometry, chip-based infusion, fraction collection and direct surface analysis into one integrated ion source platform. It allows scientists to obtain more information from complex samples than LC/MS alone.

Liquid Extraction Surface Analysis Mass Spectrometry Method for Identifying the Presence and Severity of Nonalcoholic Fatty Liver Disease

The early stages of nonalcoholic fatty liver disease (NAFLD) are characterized by the accumulation of fat in the liver (steatosis). This can lead to cell injury and inflammation resulting in nonalcoholic steatohepatitis (NASH). To determine whether lipid profiling of liver tissue can identify metabolic signatures associated with disease presence and severity, we explored liquid extraction surface analysis mass spectrometry (LESA−MS, Advion TriVersa NanoMate with LESA PLUS) as a novel sampling tool. Using LESA−MS, lipids were extracted directly from the surface of ultrathin slices of liver tissue prior to detection by high-resolution mass spectrometry (MS).

We compared the data obtained by LESA− MS to that from liquid chromatography (LC)− MS and matrix-assisted laser desorption ionization MS. Advantages of LESA− MS include rapid analysis, minimal sample preparation, and high lipid coverage.

Furthermore, since tissue slices are routinely used for diagnostics in clinical settings, LESA− MS is ideally placed to complement traditional histology. Overall LESA− MS is found to be a robust, fast, and discriminating approach for determining NAFLD presence and severity in clinical samples.