NIHR BCR Metabolomics and Lipidomics Facility, University of Cambridge

Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?

A: Almost exclusively high throughput lipid profiling of samples from large epidemiological studies, where we study gene-lifestyle interactions. The method works with plasma samples as well as dried blood spots. The method is also applied to small-scale studies of specific disease groups, dietary interventions and model systems such as yeast. The TriVersa NanoMate is also used to study the lipid composition of tissues analyzed by LESA.

Q: WHY DID YOU INCORPORATE THE TriVersaNanoMate INTO YOUR LABORATORY?

A: The TriVersa NanoMate is essential to efficiently analyze large-scale studies. It offers a really robust method for high throughput studies with minimal carryover.

Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE TriVersa NanoMate?

A: I recommend the TriVersa NanoMate to laboratories with a large number of samples requiring a robust and reliable delivery system. The TriVersa NanoMate eliminates typical nanoelectrospray ionization challenges.

Q: DO YOU HAVE ANY PUBLICATIONS OR PRESENTATIONS USING THE TriVersa NanoMate?

A: Koulman, A.; Prentice, P.; Wong, M.C.Y.; Matthews, L.; Bond, N.J.; Eiden, M.; Griffin, J.L.; Dunger, D.B., The development and validation of a fast and robust dried blood spot based lipid profiling method to study infant metabolism, Metabolomics 2014.

Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS): Examples of a New Surface Probing Technique for Clinical and Pre-Clinical Applications

Executive Summary:

  • LESA and LESAplus liquid chromatography provide novel surface analysis tools for the spatial resolution of analytes from a wide variety of surfaces.
  • Whole body or organ sections in particular can be probed for small molecules, lipids and proteins with a resolution of 400 – 1000μm.
  • Commercial solution allows for automated processing of samples (Advion TriVersa NanoMate® LESA®).

Optimization and Application of Direct Infusion Nanoelectrospray HRMS Method for Large-Scale Urinary Metabolic Phenotyping in Molecular Epidemiology

ABSTRACT: Large-scale metabolic profiling requires the development of novel economical high-throughput analytical methods to facilitate characterization of systemic metabolic variation in population phenotypes. We report a fit-forpurpose direct infusion nanoelectrospray high-resolution mass spectrometry (DI-nESI-HRMS) method with time-of-flight detection for rapid targeted parallel analysis of over 40 urinary metabolites. The newly developed 2 min infusion method requires <10 μL of urine sample and generates high-resolution MS profiles in both positive and negative polarities, enabling further data mining and relative quantification of hundreds of metabolites. Here we present optimization of the DI-nESI-HRMS method in a detailed step-by-step guide and provide a workflow with rigorous quality assessment for large-scale studies.

From the Journal of Proteome Research, Published February 2017

TriVersa NanoMate® LESA® with ESI chip® technology

The TriVersa NanoMate® LESA® is the latest in chip-based electrospray ionization technology from Advion. It combines the benefits of liquid chromatography, mass spectrometry, chip-based infusion, fraction collection and direct surface analysis into one integrated ion source platform. It allows scientists to obtain more information from complex samples than LC/MS alone.