The compound synthesis, purification, and confirmation processes often present challenges. From ensuring successful synthesis of your product, setting up a flash purification method, and finally confirming ID of fractions, the lengthy workflow can be time consuming and repetitive. This webinar will provide a foundation for flash purification processes and will showcase a novel workflow concept that breaks down the process into easy steps for optimal success. We will offer tips to optimize each essential segment of the workflow, and share seamless steps to quickly and easily drive the process from reaction monitoring to flash purification and fraction ID.
Presented by Chase Needham, Application Scientist—Flash and Prep LC
Advion Interchim Scientific. This webinar was hosted by Lab Manager and recorded 9/30/21.
Flash chromatography has traditionally used UV absorption as the main method of detection for compounds during a purification process. While UV absorption is broadly applicable to many classes of compounds, it has limited specificity to individual compounds in a mixture and misses classes of compounds that do not carry chromophores.
Mass-directed fraction collection gives users the ability to collect fractions based on mass spectrometry detection (MS) which is based on ions specific to individual compounds and provides specific molecular information. This allows for simplification in the overall purification process and greater confidence in the identity of each isolated compound. Here we describe methods of isolating natural products from green tea and turmeric powder by mass-directed fraction collection during flash chromatography and preparative LC.
This poster was presented at the 2021 ASMS national conference in Philadelphia, PA.
In this exclusive webinar, our cannabis instrumentation experts will share information about solutions that relieve the purification bottleneck for processors.
This 15-minute on-demand session will cover:
- Pesticide remediation and dewaxing
- THC remediation
- Minor cannabinoid purification
- In-house analytical
- System and Consumable options
This 159-page educational eBook is a comprehensive manual on high-throughput purification – from the theory and principles of chromatography through to method development and column selection. This valuable book offers an incredible opportunity for both beginners and experts alike to learn more about the science of chromatography and can improve your workflow and productivity. Chapter highlights include:
- Theoretical Principles (37 pages)
- Methods Development & Optimization” (45 pages) including TLC, Separative Methods transfer, Methods for Peptides purification, Injection method in Flash, Method optimisation and columns sizing
- Stationary Phases and Columns (45 pages) including Characteristics & Selection Guide, Stationary phases.
- Applications (26 pages)
- Technical Tips (15 pages)
- Troubleshooting (21 pages)
In this application note, both the purification and analytical processes for THC remediation using the Interchim ITM-5015 and Advion AVANT™ HPLC-UV systems are shown to form a complete solution for THC remediation in the hemp industry.
Successfully completing an organic synthesis involves three typically lengthy, but crucial steps: reaction monitoring, compound identification, and purification. These steps can create bottlenecks in the synthesis, unless a purpose-built, streamlined, and reliable workflow is on hand.
This whitepaper delves into some of the challenges faced by synthetic organic chemists as they navigate compound identification and purification struggles. It also describes how using a workflow that couples thin layer chromatography (TLC) and mass spectrometry (MS) to flash purification can help increase the efficiency of the entire process.
Key Objectives：
- Understand how a workflow that combines thin-layer chromatography, mass spectrometry, and flash purification can increase the speed and efficiency of an entire organic synthesis process.
- Learn about prep-free techniques that have become the new standard: scraping TLC spots, developing flash methods and performing long liquid chromatography/MS runs are not necessary with modern instrumentation that is specifically developed to take the prep-work and guess-work out of the purification process.
- Become informed about a cost-effective, user-friendly benchtop workflow solution for organic compound identification and purification that can save time and money for any lab.
The compound synthesis, purification and confirmation processes often present challenges. From ensuring successful synthesis of your product, setting up a flash purification method, and finally confirming ID of fractions, the lengthy work flow can be time consuming and repetitive.
This webinar will provide a foundation for flash purification processes, and will showcase a novel work flow concept that breaks down the process in to easy steps for optimal success. We will offer tips to optimize each essential segment of the workflow, and share seamless steps to quickly and easily drive the process from reaction monitoring to flash purification and fraction ID.
Presented by Dr. Daniel Eikel, Director of Customer Service and Product Applications, Advion.
This webinar was hosted by C&EN and recorded 8/13/20.
The combination of the Advion Interchim Scientific expression® CMS and puriFlash® flash chromatography technologies speed up the synthesis, purification and characterization of a compound.
Using the Advion Interchim Scientific expression® Compact Mass Spectrometer, the Plate Express™ TLC Plate reader, the ASAP® Atmospheric Solids Analysis Probe, and the puriFlash® XS520 flash purification system, it is now possible to execute a fast, user-friendly workflow to run TLC, harness the power of your cell phone to develop a flash method, and finally utilize a simple dipping probe to confirm fractions in seconds.
With easy sampling techniques, intuitive software, and advanced detection technology, you can easily and confidently synthesize, purify and characterize your compound, in a flash.
The compound synthesis, purification and confirmation process often presents challenges throughout. From ensuring successful synthesis of your product, setting up a flash purification method, and finally confirming ID of fractions, the lengthy work flow can be time consuming and repetitive.
In this application note, we demonstrate how the combination of Advion Interchim Scientific technologies speed up the synthesis, purification and characterization of a compound.
This series features the Advion Interchim Scientific expression® Compact Mass Spectrometer (CMS), the Plate Express™ TLC Plate Reader, the Atmospheric Solids Analysis Probe (ASAP®), and the puriFlash® XS520 flash purification system.
The Advion Interchim Scientific suite of systems and consumables allows users to harness the power of mass spectrometry, flash chromatography, prep LC and more. Simple and robust, download our brochure to learn more about:
- The Advion Interchim Scientific expression® compact mass spectrometer (CMS): A fast and easy analytical tool for the organic chemist. Ideal for fast reaction monitoring, the expression® CMS features a single quadrupole that can adapt to multiple ionization sources in seconds, including both ESI and APCI. The expression® CMS offers a variety of novel sampling techniques, including fast assay methods for liquids, solids, gases, and even air-sensitive compounds.
- Direct mass analysis of TLC plates in 30 seconds at the push of a button with Plate Express™
- One-touch analysis of solids and liquid samples with the ASAP® probe
- LC/CMS
- puriFlash® ultra performance flash purification: Ideal for method development and purification of rare and high added value compounds, the Interchim family of puriFlash® systems offer users a wide range of throughput options and the highest recovery rates at >95%.
- Mass-Guided Purification: the Interchim puriFlash® + Advion CMS offers the ideal solution for Flash-MS, and can provide fraction identification in <30 seconds.
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Fill out the form to download the full Advion Interchim Scientific brochure now.
