Dr. Jack Henion presents the possibility of using the Advion Interchim Scientific expression® Compact Mass Spectrometer (CMS) for ‘roadside breath testing’ of THC DRUID (driving under the influence of drugs) with the use of a small plastic breath collection device from Breath Explor of Uppsala, Sweden. This device serves as a simple, acceptable collection device for individuals in contrast to venous puncture with a needle or other means of collecting a biological sample.
In this ASMS 2020 Reboot presentation, you will learn:
Breath sample collection with the Breath Explor device
The potential benefits and challenges of a roadside LC/CMS testing system including an automated robot for sample preparation with the Advion Interchim Scientific expression® CMS and Advion Interchim Scientific AVANT UHPLC
The Advion LC/CMS system provides accurate and sensitive results when used to analyze breath samples for THC
Authors: Olof Beck2, Jack Henion1, Sabina Seferaj2, Peter Stamback3 1Advion, Inc., Ithaca, NY 2Karolinska Institute, Stockholm, Sweden 3Breath Explore, Munkplast AB, Uppsala, Sweden
Patulin is a mycotoxin that is a common contaminant in food and animal feed, especially in apple products. The potential health risk of patulin to humans led to the establishment of action levels in foodstuffs.
The EU, WHO and US FDA defined maximum levels of patulin in fruit juices. The EU also has specific regulations for solid apple products and foods intended for infants and young children such as apple compote.
In this application note, a simple UHPLC/CMS method using the Advion Interchim Scientific AVANT® UHPLC and expression® Compact Mass Spectrometer (CMS) system is introduced for the analysis of patulin in apple juice and apple compotes.
The research presented in this application note was a poster presentation for ASMS 2020 Reboot.
Patulin is a mycotoxin that is a common contaminant in food and animal feed, especially in apple products.The potential health risk of patulin to humans led to the establishment of action levels in foodstuffs.
The EU, WHO and US FDA defined maximum levels of patulin in fruit juices. The EU also has specific regulations for solid apple products and foods intended for infants and young children such as apple compote.
In this poster, a simple UHPLC/CMS method using the Advion AVANT UHPLC and expression Compact Mass Spectrometer (CMS) system is introduced for the analysis of patulin in apple juice and apple compotes.
This poster was presented at the ASMS 2020 Reboot.
The compound synthesis, purification and confirmation process often presents challenges throughout. From ensuring successful synthesis of your product, setting up a flash purification method, and finally confirming ID of fractions, the lengthy work flow can be time consuming and repetitive.
In this application note, we demonstrate how the combination of Advion Interchim Scientific technologies speed up the synthesis, purification and characterization of a compound.
This series features the Advion Interchim Scientific expression® Compact Mass Spectrometer (CMS), the Plate Express™ TLC Plate Reader, the Atmospheric Solids Analysis Probe (ASAP®), and the puriFlash® XS520 flash purification system.
The LS-APGD microplasma has shown potential as a combined atomic and molecular (CAM) ionization source, though to this point it has been solely interfaced with large, laboratory-scale instruments. There are many instances wherein reduced-format, lower operating overhead, analyzers are appropriate. To this end, the LS-APGD has been coupled with an Advion expressionL Compact Mass Spectrometry (CMS) for the analysis of diverse samples. This instrument has been previously travel hardened in accordance to MIL-SPEC-810, demonstrating its suitability for in-field analysis. The coupling of the LS-APGD to this instrument expands its capability for diverse analyses, with early-stage figures of merit presented here. With this new coupling, a thorough optimization of both the instrument ion optics and the LS-APGD operating parameters was necessary as this platform does not have a direct method for collisional dissociation of potentially-deleterious molecular species. Under the optimized conditions, analytical response curves were constructed for Na, Rb, Cs, and Tl, demonstrating detection limits between 17.6–146 ng mL−1 (single ng, absolute). Furthermore, CAM sampling capabilities can be realized upon changing from a 2% HNO3 to a 70 : 30 MeOH : H2O solvent system, providing molecular-level chemical information readily obtained from atomic ionization sources such as the inductively coupled plasma (ICP-MS). Here detection limits between 1–32 nM (single pg, absolute) were obtained for caffeine, acetaminophen, Δ9-tetrahydrocannabidiol, sulfadimethoxine, terfenadine, and reserpine. The unique features of the CAM ionization source on this flexible MS platform hold promise for many operation environments.
USDA-ARS, Cornell University, Michigan State University
Abstract
Processing techniques can disrupt the cotyledon cell walls of raw beans, allowing digestive enzymes greater access to intercellular stores of micronutrients such as iron during digestion. This study evaluated the iron bioavailability of seven bean varieties with different seed coat colors (white, yellow, cranberry, red, black) either boiled or processed into spaghetti pastas formulated from heat treated bean flour as the major ingredient (90% bean flour). Iron bioavailability was significantly (p ≤ 0.05) higher in spaghetti made from white or yellow bean varieties Snowdon, Alpena, Samurai and Canario when compared to boiled beans. Although cotyledon cells were broken and the phytate to iron molar ratios were significantly lower, the iron bioavailability of the cranberry (Etna), red kidney (Red Hawk) and black (Zenith) bean varieties did not improve after processing into spaghetti. Iron bioavailability of bean-based pastas was associated with procyanidin and cinnamtannin compounds that have a negative impact on the absorption of iron.
Analysis was performed by LC/MS using the Advion Interchim Scientific expression® Compact Mass Spectrometer (CMS).
The combination of the Advion Interchim Scientific® expression® CMS puriFlash® flash chromatography technologies speed up the synthesis, purification and characterization of a compound.
Kyungpook National University, Dankook University, Ulsan National Institute of Science and Technology, South Korea; Icahn School of Medicine at Mount Sinai, United States
Abstract
Sphingosine kinase1 (SphK1) is an acetyl-CoA dependent acetyltransferase which acts on cyclooxygenase2 (COX2) in neurons in a model of Alzheimer’s disease (AD). However, the mechanism underlying this activity was unexplored. Here we show that N-acetyl sphingosine (N-AS) is first generated by acetyl-CoA and sphingosine through SphK1. N-AS then acetylates serine 565 (S565) of COX2, and the N-AS-acetylated COX2 induces the production of specialized pro-resolving mediators (SPMs). In a mouse model of AD, microglia show a reduction in N-AS generation, leading to decreased acetyl-S565 COX2 and SPM production. Treatment with N-AS increases acetylated COX2 and N-AS-triggered SPMs in microglia of AD mice, leading to resolution of neuroinflammation, an increase in microglial phagocytosis, and improved memory. Taken together, these results identify a role of N-AS in the dysfunction of microglia in AD.
Mass analysis was carried out using an Advion expression® Compact Mass Spectrometer (CMS).
In this application note, the Advion Interchim Scientific expression® Compact Mass Spectrometer (CMS) with volatile APCI (vAPCI) is used to analyze volatile compounds present during the fermentation of homebrewed beer by Stephanie Rankin Turner of Loughborough University.
The complex flavor of beer is primarily a result of the ingredients used, the brewing method, and conditions during fermentation. The analysis of beer throughout this process can be invaluable. Being one of the most widely consumed beverages worldwide, rapid and reliable analytical techniques are essential to keep up with demand and production.
This note demonstrates the use of the Advion Interchim Scientific expression® CMS with vAPCI for the analysis of volatile compounds from the headspace of homebrew beer and hops. The Venturi-assisted interface of the instrument enabled rapid sampling of volatiles, allowing the changing volatile profile of the homebrew to be observed throughout the fermentation process. This simple method would be suitable for fast quality control during alcoholic beverage production.
National Organization for Drug Control and Research, Cairo University
Abstract
Stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) and normal-phase high-performance thin-layer chromatography (NP-HPTLC) methods have been developed for the determination of palonosetron which is a potent antiemetic drug used with chemotherapy. Forced degradation studies were performed on palonosetron to study its stability behavior. The drug was subjected to acid, base, neutral hydrolysis, oxidation, thermal and photolytic conditions. Mass analysis has been performed to elucidate the oxidative degradants by Advion compact mass spectrometer. HPLC separation was achieved on PerfectChrom 100 C4 (250 × 4.6 mm, 5 μm particle size) column using buffer (20 mM dipotassium hydrogen orthophosphate, adjusted with phosphoric acid to pH 2.5):acetonitrile:methanol (60:30:10, v/v) as the mobile phase with isocratic mode at a flow rate of 1 mL/min using photodiode array detector (PAD) at 210 nm. The method showed adequate sensitivity concerning linearity, accuracy and precision over the range of 0.1–10 μg/mL. Limit of detection (LOD) and limit of quantification (LOQ) were determined to be 0.03 μg/mL and 0.09 μg/mL, respectively. HPTLC separation was carried out on aluminum plates pre-coated with silica gel 60 F254 using methanol:ammonia (10:0.5, v/v) as the mobile phase. CAMAG scanner was operated at 254 nm for the densitometric measurement in the absorbance mode. A polynomial relationship was constructed in concentration range of 0.1–2 μg/band, with LOD and LOQ 0.02 μg/band and 0.06 μg/band, respectively. The cited chromatographic methods were successfully applied to the determination of palonosetron in the presence of its degradation products and additives in the commercially available vials. Method validation was performed as per the ICH guidelines confirming methods robustness to be used in quality control laboratories. Statistical comparisons have been performed between the results of the cited chromatographic methods and those of the official one using Student’s ttest and F test values at 95% confidence interval level, revealing good accuracy and precision.
