Supercritical fluid chromatography (SFC) is a fast growing technique. Initially used almost exclusively for only thermally labile and chiral compounds it is now becoming accepted as a versatile analytical technique applied across a broad range of compounds. It is faster and higher-throughput than regular HPLC and Preparative LC and becoming popular as a ‘green’ technique, with far lower solvent consumption and lower consumable cost than HPLC. SFC is also compatible with a wide range of detection methods including electrospray (ESI) and atmospheric pressure chemical ionization (APCI) mass spectrometry.
Download this product note to see how SFC combined with compact mass spectrometry (CMS) offers chemists several benefits.
Goa College of Pharmacy
Abstract
The present study was undertaken to carry out phytochemical investigation of the ethanolic extract of the stem bark of Terminalia tomentosa Roxb (ex DC) Wight & Arnbelonging to the family Combreteaceae. The plant is known in Sanskrit as Asana, in English as Black murdah, in Hindi as Asan, Saj, Sain and in Marathi as Ain.The plant has been known to possess various pharmacological activities like antifungal, antioxidant, antihyperglycaemic, antidiarrhoeal, antileucorrheal etc. The bark of the plant is astringent& useful in ulcers, vata, fractures, haemorrhages, bronchitis, diarrhoea etc.Preliminary phytochemical screening of the ethanolic extract of stem bark revealed the presence of carbohydrates, flavonoids, triterpenoids,steroids, tannins and saponins. The chemical entities isolated and characterised includes 4 – methy l – 4 – hydroxymethylene – 6β – (10 – methyl octanyl) cyclohexane (Arjuna homoses quiterpenol), di-n-octyl phthalate, di isobutyl phthalate and dibutyl phthalate. All these phytoconstituents are reported for the first time from the ethanolic extract of stem bark of T.tomentosa.
Mass spectra was obtained by ESI ionization using the Advion expression Compact Mass Spectrometer (CMS).
In this study we evaluated the performance of a Preparative SFC‐MS system combining the JASCO Preparative SFC with the Advion expression® CMS (Compact Mass Spectrometer) utilizing a simplified passive splitter for mass‐directed fraction collection. The initial test explored a wide range of flow rates both isocratic and gradient to test performance and reliability of the passive splitter, coinciding timing of MS and UV signal and the clearance of the source with highly concentrated samples.
The expression CMS was designed by expert mass spectroscopists and chemists to create the ideal platform for scientists to get the most out of their research. Full integration (control, data acquisition, and analysis) with Agilent Technologies’ LC systems allows users a streamlined approach for their LC/CMS methods.
There is a concerted effort by regulatory bodies, such as the EPA to reduce the exposure of environmental contaminants, such as inorganic anions to humans and the environment.
Achieving lower analytical detection limits of hazardous compounds in environmental matrices such as water, soils and sediments allows regulatory bodies to make better informed decisions on the degree of contamination of an environmental system as well as the remedial actions required to limit adverse health and environmental effects.
Mass spectrometry provides the tool to achieve lower detection limits and greater selectivity when compared to conductivity and UV detectors.
In natural product research the isolation of compounds from crude extracts is a key element. An increase in the efficiency of the purification process, by improvement of instrumental and methodological approaches, remains a crucial point. In this respect, modern flash chromatography, has evolved and uses 15 μm spherical particles for efficient separation at moderate pressures. Flash C-18 was tested for the rapid isolation of various plant secondary metabolites of interest.
There must be better ways to do sample preparation for food matrices! And with good sample prep better analyses occur. It is human nature to resist change. Let us embrace new ways of doing things if they can be shown to be better, faster and easier.
A novel compact single quadrupole mass spectrometer for hood-based applications in the analysis of chemical reactions.
Medical chemists are routinely faced with personally over a hundred new chemical entities (NCE’s) each year for testing as future pharmaceutical drug candidates. The goal is to prepare a high yield, relatively pure product via an optimized synthetic route.
Advion demonstrates a novel compact single quadrupole mass spectrometer for hood-based applications in the analysis of chemical reactions employing either LC/MS or flow injection analysis (FIA).
