The CheMS user interface simplifies the mass spectrometry experience and makes it more accessible in chemistry and biochemistry laboratories. This allows the chemist to obtain optimal data without needing to be an expert in mass spectrometry.
Available with the Advion expression® compact mass spectrometer (CMS) is a fast and easy tool that is ideal for the teaching environment — Students simply select an ionization method for effortless instrument setup at the click of a button.
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N. Holmes, G.R. Akien, R.J.D. Savage, C. Stanetty, I.R. Baxendale, A.J. Blacker, B.A. Taylor, R.L. Woodward, R.E. Meadows, and R.A. Bourne
An automated continuous reactor for the synthesis of organic compounds, which uses online mass spectrometry (MS) for reaction monitoring and product quantification, is presented. Quantitative and rapid MS monitoring was developed and calibrated using HPLC. The amidation of methyl nicotinate with aqueous MeNH2 was optimised using design of experiments and a self-optimisation algorithm approach to produce >93% yield.
Using Thin-Layer Chromatography with Compact Mass Spectrometry
A sprig of mistletoe symbolizes a tradition of romance; however, reputed to be the “kiss of death,” mistletoe is said by some to be so poisonous that humans can be killed if they ingest the leaves or berries.
In the spirit of the Holiday season and to ensure that mistletoe kisses are enjoyed and are ‘non-toxic’, Nigel Sousou and Jack Henion employed the Advion TLC/CMS system to analyze a commercial Tincture of Mistletoe ethanolic extract to determine whether tyramine is present in the extract of mistletoe.
A: Our exclusively undergraduate program in chemistry is fortunate to have a nice inventory of analytical instrumentation for use in various lab courses and student/faculty research. As the department’s analytical chemist, I coordinate the use of these instruments and the development of laboratory projects for use in our curricular labs. I also direct a group of undergraduate student assistants in research aimed at developing new devices and methodologies for microscale spectroelectrochemical measurements.
Q: WHAT WAS YOUR PREVIOUS WORK FLOW OR CHALLENGES?
A: Our department’s mass spectrometry instrumentation (two GCMS systems and an ambient pressure gas analyzer) was limited to EI ionization of gaseous and volatile liquid samples until we purchased the Advion CMS.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: Our faculty agreed the need existed to incorporate additional MS capabilities to our instrumentation holdings for both our undergraduate lab curriculum and several of our faculty members’ research efforts. The Advion CMS was an ideal choice with regard to cost, portability and flexibility. In particular, the ASAP probe available for APCI mode was an attractive feature supporting our development of lab projects with forensic and pharmaceutical emphasis.
Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE EXPRESSION CMS?
A: I think the Advion system is well suited for a variety of undergraduate curricular and research lab applications.
Q: DO YOU HAVE ANY PUBLICATIONS OR PRESENTATIONS USING THE EXPRESSION CMS?
A: None to report yet, but I’m optimistic – at present, a student in my instrumental analysis course is satisfying her independent project requirement by working with the ASAP probe to develop a simple internal standard approach to permit quantitation of components in pharmaceutical preparations.
Q: What is the focus of your lab’s research? A: Our research is focused on developing and exploiting chemical tools and principles to address questions in plant and microbial carbohydrate biochemistry. Projects typically involve some combination of chemical and enzymatic synthesis, inhibitor design and assessment, biochemistry, structural biology and omics analyses.
Q: What was your previous workflow or challenges? A: We synthesize a lot of different molecules in the lab, many of which have relatively low molecular weights. To acquire low resolution MS spectra of purified compounds we relied on MALDI-TOF or on direct injection into an expensive LC-MS system (ESI). Access to these instruments required booking in advance and there was also often a delay in obtaining results.
Q: Why did you incorporate the expression® CMS into your lab? A: We wanted an instrument that would allow rapid access to low resolution MS data for low molecular weight compounds. Immediate access to this instrument allows rapid confirmation of product formation in chemical and ezymatic syntheses; the Compact Mass Spectrometer (CMS) is particularly useful for rapid identification of analytes from LC separations. All of this can be done by direct injection but in our lab the injection from a TLC silica plate using the Advion Interchim Scientific Plate Express™ is being increasingly used as a faster alternative. The APCI ion source is another great capability of the CMS, particularly for analysis of lipophilic compounds such as triterpenes, which we have experienced difficulties in analysing by other ionisation techniques.
Q: Who would you recommend to purchase the expression® CMS? A: Any group working with organic molecules that requires fast access to low resolution MS spectra. We would especially recommend coupling the expression® CMS with the Plate Express™ for labs that routinely use TLC for monitoring chemical syntheses and biotransformations.
We have developed a modular software system that enables researchers to monitor and control chemical reactions via the Internet, using any device from any location in the world. It facilitates the automation of synthetic procedures and is able to autonomously self-optimize reaction parameters to find the best conditions meeting customizable, multicomponent optimization functions. In this report, we demonstrate its utility as applied to reaction automation to maximize the output from a fixed volume of catalyst. We also showcase its ability to optimize a three-dimensional heterogeneous catalytic reaction and a five-dimensional Appel reaction against various target functions.
In this publication, the Advion expression Compact Mass Spectrometer (CMS) was paired with a flow reaction system for simple reaction monitoring.
An office colleague has stolen a prized chocolate bar, and Dr. Eikel with the help of his partner, Dr. Henion, use an Atmospheric Solids Analysis Probe (ASAP) enabled expression CMS to determine whodunnit. This application note describes the process, and points the finger at the guilty culprit!
This note covers work carried out at Leeds University with co-workers from Durham University in the area of flow chemical synthesis using the expression CMS (Advion, Ithaca, NY). Two different reactions are described.
P. P. Hurtado1, E., Palmer1, C. Aldcroft2, H. Moore2, A. Baker2. M. Allen2, J. Jones2, M.A. Turner1, J.C. Reynolds1
1Centre for Analytical Science, Department of Chemistry, Loughborough University, Ashby Road, Loughborough, Leicester, LE11 3TU, UK 2Advion UK Ltd
The development of new interfaces that enable direct analysis of VOCs represents an attractive and low cost alternative for rapid analysis. For example, the food industry is particularly interested in this type of analysis, where the freshness of different foods can be rapidly measured by the release of its volatiles. Numerous techniques have been used for this purpose. However, mass spectrometry remains the gold standard technique enabling chemical specificity of detection.
A: The Liverpool School of Tropical Medicine (LSTM) is dedicated to research and teaching on tropical diseases. We are helping improve the health of some of the poorest people in the world by carrying out scientific research and developing treatments that will directly benefit them.
Specifically, I work in the department of parasitology, where our focus is on the key tropical parasites such as malaria, TB and filarial worms.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: The expression was originally purchased to save us from a 15-minute walk to get simple MS data at the University’s main analytical service. Also, it is compact and very easy to use, you do not need to be an expert in MS to generate very useful and meaningful data.
Q: HOW DO YOU USE THE EXPRESSION CMS?
A: We use the expression in the direct injection configuration for checking whether reactions have worked and whether the next purification and/or reaction steps are worth proceeding.
Before we got the expression, we either had to walk across campus to use the central service or continue without any knowledge as to whether our reactions had worked. The expression saves us time and boosts our overall research performance.
I am also planning to use it for some simple metabolism studies in the near future.
