Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: Our work is focused on the development and implementation of chromatographic purification processes, ranging from mg to 100 kg scale. Many compounds of interest lack chromophores and cannot be detected by classical UV detection.
Q: WHAT WAS YOUR PREVIOUS WORKFLOW AND EXPERIENCED CHALLENGES?
A: In the past, the fraction collection during preparative HPLC was very time consuming as we had no indicator when the compound of interest was eluting in the chromatographic process in case it was not UV-active. All fractions had to be analyzed by TLC and in many cases additionally with HPLC and Corona detection before we could identify the fractions of interest.
Q: WHY DID YOU INCORPORATE THE EXPRESSION® CMS INTO YOUR LABORATORY?
A: We use the Advion Interchim Scientific expression® CMS as a robust and versatile detector in our chromatographic processes, both MPLC and HPLC. The data transfer into the control software of our prep HPLC systems was straightforward. The Advion MS is shared with our R&D department for more conventional applications and gave both departments very quick access to MS data. Both departments appreciated the quick set-up, the user-training, and the reliable after sales service whenever a question pops up.
Q: TO WHOM WOULD YOU RECOMMEND THE EXPRESSION® CMS?
A: The Advion MS is a robust instrument that can be used in different environments. It does not require a lot of training and is easy to understand. Whenever you have a need for MS data, be it online or offline, Advion MS might give you very easy access to the data you need.
Overview:
Direct sample analysis is possible via an Atmospheric Solids Analysis Probe (ASAP®) combined with atmospheric pressure chemical ionization (APCI) on a compact mass spectrometer. This technique allows for the rapid investigation (<30 sec) of material suspected of containing trace amounts of explosive residues.
The expression® Compact Mass Spectrometer (CMS) with an Atmospheric Solids Analysis Probe (ASAP®) is a high performance instrument with a small footprint that allows for efficient liquid and solid screening at the point of need. The CMS provides rapid and accurate detection with significantly reduced false-positives and false-negatives than other techniques.
Here, we demonstrate the use of the ASAP® probe and expression® CMS detector for explosives screening. Sampling via the ASAP® probe is then inserted in to the APCI source of the mass spectrometer, initiating in a fast ionization reaction that provides rapid results.
Bacteria can be benign, beneficial and harmful, for more than 70 years we have been combatting these microscopic creatures with antibiotics. Increasingly though strains of antimicrobial resistant bacteria are becoming commonplace. This has motivated numerous studies aimed at deciphering the evolution of antibiotic resistance. Volatile Organic Compounds (VOCs) are ubiquitous in nature and are produced through various metabolic and catabolic processes. The profile of bacterial VOCs could therefore offer an insight into the detection of the type of bacteria and how they grow. This research utilizes a Volatile Atmospheric Pressure Chemical Ionization (vAPCI) source coupled to a Advion CMS instrument for detection of bacterial VOCs from in-vitro samples. This rapid and non-invasive approach of sampling VOCs and the implementation of a metabolomics workflow discriminates between E.coli and staphylococcus aureus and demonstrates how bacterial profiles can change over time.
Many chemical reactions carried out by synthetic chemists involve air-sensitive compounds such as metal catalysts and organometallics and must therefore be carried out in a glove box or using a Schlenk line to prevent oxidation and hydrolysis. Sampling and transportation of these samples to a mass spectrometer by an Atmospheric Solids Analysis Probe (ASAP®) without exposure to the air presents a problem.
Here, the inert ASAP probe (iASAP), a modification of the technique developed by Professor Ingo Krossing’s group at the Albert-Ludwigs-University of Freiburg, allows sampling and transportation in an environment of inert gas to prevent decomposition of the compound.
The expression® CMS coupled with the Atmospheric Solids Analysis Probe (ASAP®) is used to determine purity and detect adulterants in coconut oil. In this application note, pure coconut oil is tested against a sample of coconut oil adulterated with canola oil for comparative data analysis and a fast and easy method for purity analysis.
Shared live at the 254th ACS National Meeting in Washington, D.C. –
John Matson, Ph. D., Matson Research Group on Macromolecular and Supramolecular Chemistry, Assistant Professor, Virginia Tech.
Listen to the recorded workshop session highlighting the role of H2S in the human body and how compact mass spectrometry is used to assay peptides via direct injection for reaction monitoring, and coupled with HPLC for purification.
Many chemical reactions carried out by synthetic chemists involve air-sensitive compounds such as metal catalysts and organometallics and must therefore be carried out in a glove box or using a Schlenk line to prevent oxidation and hydrolysis. Sampling and transportation of these samples to a mass spectrometer by an Atmospheric Solids Analysis Probe (ASAP ®) without exposure to the air presents a problem. Here, the inert ASAP probe (iASAP), a modification of the technique developed by Professor Ingo Krossing’s group at the Albert-Ludwigs-University of Freiburg, allows sampling and transportation in an environment of inert gas to prevent decomposition of the compound.
Introducing a new type of mass spectrum, available exclusively in the Advion Peak Express™ software suite: the Delta Spectrum (ΔS). The ΔS is available with the expression® compact mass spectrometer (CMS) to look beyond chemical noise and automatically detect even the smallest peaks without knowing the m/z in advance.
With the Peak Express™ software featuring ΔS, you can:
- Obtain clear detection of peaks without knowing their mass
- Find adducts, dimers, fragments, side-reactions and other unexpected compounds at the click of a button, in real-time or with post-processing
- Highlight minor components in complex mixtures and dirty matrices
- Control mass-directed purification without providing the compound mass
- Acquire XIC-quality data while scanning the entire mass range, allowing Peak Express™ to tell you the m/z
Advion Interchim Scientific’s range of AVANT®, high performance, liquid chromatography systems can be used standalone with UV and UV/Vis detector options, or with the expression® compact mass spectrometer to provide seamlessly integrated LC/CMS under the full control of Advion Interchim Scientific’s simple, intuitive Mass Express software suite.
Modular, stackable design, with many options, provides custom solutions for both HPLC and UHPLC needs. From the simplest manual injection HPLC to a fully automated, streamlined UHPLC system and everything in-between, the AVANT® series can be configured to fit your analytical requirements and your budget.
TLC/CMS, the atmospheric liquids and solids probe (ASAP), and the volatile APCI source provide direct analysis without sample preparation – even for air-sensitive compounds.
With the daily need to analyze a variety of samples, these techniques are indispensable in a busy lab.
Learn how a single instrument can be adapted to each of these sample requirements and rapidly changed to accommodate back-to-back assays. Listen to users in the field speak about the innovative sample inlets that have cut down on sample prep and streamlined their everyday work flow.
During this webinar you will:
- Learn how the CMS offers real-time results and decision making right at the bench. This allows chemists to optimize reactions, know when to quench, and know when they have failed and to move on
- Learn several novel sample techniques that can streamline workflow in your lab
- Find fast analysis methods for liquids, solids and vapor-phase compounds
Hear from leaders in mass spectrometry including:
- Dr. Jack Henion, Advion Scientific Founder
- Matthew Turner, Experimental Officer in Mass Spectrometry, Department of Chemistry, Loughborough University
- Tao Yongfeng, Post Doctorate, The Romo Group, Baylor University
- Sean M. Kerwin, PhD, Associate Professor, Department of Chemistry & Biochemistry, Texas State University
