Many chemical reactions carried out by synthetic chemists involve air-sensitive compounds such as metal catalysts and organometallics and must therefore be carried out in a glove box or using a Schlenk line to prevent oxidation and hydrolysis. Sampling and transportation of these samples to a mass spectrometer by an Atmospheric Solids Analysis Probe (ASAP ®) without exposure to the air presents a problem. Here, the inert ASAP probe (iASAP), a modification of the technique developed by Professor Ingo Krossing’s group at the Albert-Ludwigs-University of Freiburg, allows sampling and transportation in an environment of inert gas to prevent decomposition of the compound.
Peak Express™: Software for the Synthetic Organic Chemist
Introducing a new type of mass spectrum, available exclusively in the Advion Peak Express™ software suite: the Delta Spectrum (ΔS). The ΔS is available with the expression® compact mass spectrometer (CMS) to look beyond chemical noise and automatically detect even the smallest peaks without knowing the m/z in advance.
With the Peak Express™ software featuring ΔS, you can:
- Obtain clear detection of peaks without knowing their mass
- Find adducts, dimers, fragments, side-reactions and other unexpected compounds at the click of a button, in real-time or with post-processing
- Highlight minor components in complex mixtures and dirty matrices
- Control mass-directed purification without providing the compound mass
- Acquire XIC-quality data while scanning the entire mass range, allowing Peak Express™ to tell you the m/z
Advion Interchim Scientific’s AVANT® HPLC & UHPLC systems, providing simple, high-performance LC/CMS with the expression® CMS
Advion Interchim Scientific’s range of AVANT®, high performance, liquid chromatography systems can be used standalone with UV and UV/Vis detector options, or with the expression® compact mass spectrometer to provide seamlessly integrated LC/CMS under the full control of Advion Interchim Scientific’s simple, intuitive Mass Express software suite.
Modular, stackable design, with many options, provides custom solutions for both HPLC and UHPLC needs. From the simplest manual injection HPLC to a fully automated, streamlined UHPLC system and everything in-between, the AVANT® series can be configured to fit your analytical requirements and your budget.
Mass Spectrometry for Chemists: Direct Analysis of TLC Plates, Solids and Gases
TLC/CMS, the atmospheric liquids and solids probe (ASAP), and the volatile APCI source provide direct analysis without sample preparation – even for air-sensitive compounds.
With the daily need to analyze a variety of samples, these techniques are indispensable in a busy lab.
Learn how a single instrument can be adapted to each of these sample requirements and rapidly changed to accommodate back-to-back assays. Listen to users in the field speak about the innovative sample inlets that have cut down on sample prep and streamlined their everyday work flow.
During this webinar you will:
- Learn how the CMS offers real-time results and decision making right at the bench. This allows chemists to optimize reactions, know when to quench, and know when they have failed and to move on
- Learn several novel sample techniques that can streamline workflow in your lab
- Find fast analysis methods for liquids, solids and vapor-phase compounds
Hear from leaders in mass spectrometry including:
- Dr. Jack Henion, Advion Scientific Founder
- Matthew Turner, Experimental Officer in Mass Spectrometry, Department of Chemistry, Loughborough University
- Tao Yongfeng, Post Doctorate, The Romo Group, Baylor University
- Sean M. Kerwin, PhD, Associate Professor, Department of Chemistry & Biochemistry, Texas State University
A metabolite profiling method for diagnosis of precancerous cervical lesions and HPV persistence
The data collected from the analysis on the portable Advion CMS were treated in exactly the same way as the data collected from the Waters LCT and although the mass resolution and sensitivity of the two instruments is different the same clustering patterns were seen in the PCA scores plots. The PCA loadings plots also showed the same patterns with the same major 385.4 mass bin responsible for the separation of the classes seen in the scores plots.
The findings are important because they suggest that metabolite profiling could offer an accurate, and time-effective tool for identifying women at increased risk of HPV persistence. Current screening for cervical cancer risk relies, as a first step, on a cytological assessment which can be difficult to classify at the low-grade end of cervical cell abnormality and currently there is no screening tool for predicting HPV persistence. Metabolite profiling therefore has the potential to improve existing screening methods for cell abnormality and to supplement information about HPV positivity; the critically important risk factor for cervical cancer.
From Bioanalysis, Published May 2017
Real-time monitoring of exhaled volatiles using atmospheric pressure chemical ionization on a compact mass spectrometer
Aim: Breath analyses have potential to detect early signs of disease onset. Ambient ionization allows direct combination of breath gases with MS for fast, on-line analysis. Portable MS systems would facilitate field/clinic-based breath analyses.
Results & methodology: Volunteers ingested peppermint oil capsules and exhaled volatile compounds were monitored over 10 h using a compact mass spectrometer. A rise and fall in exhaled menthone was observed, peaking at 60–120 min. Real-time analysis showed a gradual rise in exhaled menthone postingestion. Sensitivity was comparable to established methods, with detection in the parts per trillion range.
Conclusion: Breath volatiles were readily analyzed on a portable mass spectrometer through a simple inlet modification. Induced changes in exhaled profiles were detectable with high sensitivity and measurable in real-time.
From Bioanalysis, Published June 2016
Delta Ion Current (ΔIC)- A novel approach to plot total ion currents enhancing the signal from newly detected ions
The novel Peak Express™ technology was evaluated and applied to natural product extracts.
Peak Express™ utilizes delta ion currents, a differential ion current with significantly improved signal-to-noise as well as enhanced detection ability for low abundance compounds.
Identication of an unknown biocide by volatile atmospheric pressure chemical ionization (vAPCI) and compact mass spectrometry (CMS)
A rapid unknown sample analysis is performed using a novel volatile APCI source with CMS detection and on-line database search.
Compact mass spectrometer (CMS) analysis of uniformly 13C-labeled amino acids to identify authentic therapeutic drugs and protect against counterfeits
Cost effective, multidimensional GRAS (generally recognized as safe) counterfeit protection of drugs based on uniformly 13C labeled amino acids with compact mass spectrometry detection.
Synthesis and full characterization of an iridium B–H activation intermediate of the monocarba-closo-dodecaborate anion
The preparation and full characterization of an iridium complex of the monocarba-closo-dodecaborate anion is reported. It was prepared by B–H bond activation using a tosyl amide directing group.
Analysis by spectroscopic methods and X-ray crystallography revealed the presence a direct B–Ir interaction. The carborane acts as aB,N chelating ligand towards the Ir(Cp*)(solvent) fragment, resulting in a monomeric complex that is inert in solution and the solid state.
Treatment with N-chlorosuccinimide resulted in selective monochlorination of the B–Ir position. In addition, its structure, spectroscopic features and reactivity were investigated by DFT calculations.