Oligonucleotides have gained significant attention in biopharmaceutical development due to their ability to modulate gene or protein expression. This application note aims to demonstrate the HPLC/UV analysis of multiple oligo samples and utilize HPLC/CMS analysis to determine their molecular weight.
Purification of Bioactive Peptides from Complex Mixtures using the puriFlash® 5.250 PrepLC and expression® CMS Detector
In this application note, we use whey protein isolate and two casein macro peptides as an example to demonstrate the isolation and purification of bioactive peptide components with a system comprised of a puriFlash® 5.250 prepLC coupled online to an expression® CMS detector.
A Handheld Guide: Fun & Easy Mass Spectrometry Experiments for your Teaching Lab
A book of experiments for your students
The expression® CMS (Compact Mass Spectrometer) is the ideal instrument for the teaching lab thanks to its small size, ease of use, and one-click software. This pocket guide of classroom experiments offers students the ability to gain hands-on instrument experience by eliminating complex and difficult sample preparation.
The Preparation, Confirmation, and Quantitation of the Ionic Imaging Contrast Agent, Gd(III)-DOTA
In this application note, we demonstrate a complete solution for the synthesis, purification, and quantitation of Gd(III)-DOTA, an ionic imaging contrast agent with better chemical stability and lower toxicity compared to the currently used Gd-(DTPA).
Analysis of Iohexol using the Advion Interchim Scientific® AVANT® HPLC and expression® CMS System
Iohexol is a widely used non-ionic imaging agent that improves contrast for x–ray analysis. Its low osmolality allows for a rapid clearance via the kidney, preventing reabsorption and further metabolization. This makes iohexol a compound with a better safety profile compared to other imaging agents.
In this application note, a simple and accurate HPLC-CMS method for iohexol analysis is introduced.
Rapid Analysis Using Direct Microextraction Assays Coupled to the New SOLATION® ICP-MS
Recorded February 2, 2023: LIVE Location, the 2023 European Winter Conference on Plasma Spectrochemistry
Speakers:
Janez Ilaš, Ph. D., Professor, University of Ljubljana
Fadi Abou-Shakra, Director of Advion Interchim Scientific® ICP-MS Portfolio
Join Advion Interchim Scientific® with guest speaker from the University of Ljubljana, Faculty of Pharmacy, to learn more about a new novel sample introduction method for ICP-MS utilizing direct analysis via microextraction. First you will learn microextraction methods with the Plate Express TLC Plate/microextraction reader coupled to the single-quadrupole expression® Compact Mass Spectrometer (CMS). After understanding the basics of the platform, you will learn more about how this technology was reimagined to suit the SOLATION® ICP-MS for a rapid method to determine isotopic analysis faster and easier than ever before.
Ready to learn more?
Streamlined Benchtop Mass Spectrometry for the Pharmaceutical Industry
Maximize your analytical spectroscopy workflow in pharmaceutical applications—even with limited space at the bench. This session features innovative solutions for streamlining everyday needs in the lab, including reaction monitoring utilizing fast, prep-free sample introduction techniques for answers in seconds. You will also learn the latest technology in heavy metal analysis, as well as purification tools to maximize your scale-up efforts.
As a viewer, you will learn more about:
- How to obtain direct mass analysis from TLC plates in seconds
- Prep-free mass sampling techniques such as a direct analysis probe
- How heavy metal analysis technology has improved for maximum use in the pharmaceutical lab
- How scale-up needs can be met with the latest purification technologies
The Extraction, Identification, Purification and Quantitation of Cyanidin-3-glucoside in Black Rice
In this application note, we show how Cyanidin-3-glucoside can be extracted from black rice and purified with a prepLC/Flash system. The amount and purity of Cyanidin-3-glucoside was measured utilizing a certified reference standard.
Extraction, Identification and Purification of (-)- Epigallocatechin Gallate From Green Tea
In this application note, a method to separate and purify green tea catechins with preparative liquid chromatography (Prep-LC) using the puriFlash® 5.250 system is demonstrated. An additional HPLC-UV-MS method is also demonstrated for compound confirmation and purity analysis.
Extraction and Purification of 3 Curcuminoids from Turmeric Powder
In this application note, a method to separate and purify 3 curcuminoids from turmeric powder using thin layer chromatography (TLC) with mass spectrometry and flash chromatography is demonstrated.