In this application note, we compare the use of a flash chromatography system with a UV detector alone versus the same system paired with a mass spectrometer. The data highlights the impact on workflow efficiency and underscores the importance of selecting the appropriate splitter.
The specificity of MS detection improves the efficiency of the purification workflow (compared to traditional Flash and HPLC).
In this application note, scientists from GEOMAR Helmholtz Centre for Ocean Research Kiel research residual TNT in the water phase from dumped weapons throughout the southwestern Baltic Sea. The Advion Interchim Scientific® expression® Compact Mass Spectrometer (CMS) is used for real-time sample detection of explosive chemicals released from these underwater relic munitions.
How it Works Webinar
Please join us for an insightful webinar where we’ll focus our discussion on the qualitative analysis of oligonucleotides using the HPLC-UV/MS method. Discover how this approach can offer you purity analysis, and molecular weight determination through charge deconvolution, etc., and gain access to valuable tricks and tips for optimizing oligonucleotide analysis. Additionally, learn how a streamlined method can provide both UV and MS analysis in a single run.
Speaker:
Changtong Hao
Senior Applications Scientist
Advion Interchim Scientific®
This Webinar was originally presented November 9, 2023 on LabRoots.com
ABSTRACT
In this webinar, you’ll learn how to address your lab’s purification pain-points with methods and instrumentation to maximize your budget, time and increase overall efficiency. During this session you will discover:
SPEAKER
Chase Needham, puriFlash and PrepLC Applications Scientist, Advion Interchim Scientific®
Oligonucleotides have gained significant attention in biopharmaceutical development due to their ability to modulate gene or protein expression. This application note aims to demonstrate the HPLC/UV analysis of multiple oligo samples and utilize HPLC/CMS analysis to determine their molecular weight.
In this application note, we use whey protein isolate and two casein macro peptides as an example to demonstrate the isolation and purification of bioactive peptide components with a system comprised of a puriFlash® 5.250 prepLC coupled online to an expression® CMS detector.
The expression® CMS (Compact Mass Spectrometer) is the ideal instrument for the teaching lab thanks to its small size, ease of use, and one-click software. This pocket guide of classroom experiments offers students the ability to gain hands-on instrument experience by eliminating complex and difficult sample preparation.
In this application note, we demonstrate a complete solution for the synthesis, purification, and quantitation of Gd(III)-DOTA, an ionic imaging contrast agent with better chemical stability and lower toxicity compared to the currently used Gd-(DTPA).
Iohexol is a widely used non-ionic imaging agent that improves contrast for x–ray analysis. Its low osmolality allows for a rapid clearance via the kidney, preventing reabsorption and further metabolization. This makes iohexol a compound with a better safety profile compared to other imaging agents.
In this application note, a simple and accurate HPLC-CMS method for iohexol analysis is introduced.
Recorded February 2, 2023: LIVE Location, the 2023 European Winter Conference on Plasma Spectrochemistry
Speakers:
Janez Ilaš, Ph. D., Professor, University of Ljubljana
Fadi Abou-Shakra, Director of Advion Interchim Scientific® ICP-MS Portfolio
Join Advion Interchim Scientific® with guest speaker from the University of Ljubljana, Faculty of Pharmacy, to learn more about a new novel sample introduction method for ICP-MS utilizing direct analysis via microextraction. First you will learn microextraction methods with the Plate Express TLC Plate/microextraction reader coupled to the single-quadrupole expression® Compact Mass Spectrometer (CMS). After understanding the basics of the platform, you will learn more about how this technology was reimagined to suit the SOLATION® ICP-MS for a rapid method to determine isotopic analysis faster and easier than ever before.
Ready to learn more?