How it Works Webinar
Please join us for an insightful webinar where we’ll focus our discussion on the qualitative analysis of oligonucleotides using the HPLC-UV/MS method. Discover how this approach can offer you purity analysis, and molecular weight determination through charge deconvolution, etc., and gain access to valuable tricks and tips for optimizing oligonucleotide analysis. Additionally, learn how a streamlined method can provide both UV and MS analysis in a single run.
Speaker:
Changtong Hao
Senior Applications Scientist
Advion Interchim Scientific®
Oligonucleotides have gained significant attention in biopharmaceutical development due to their ability to modulate gene or protein expression. This application note aims to demonstrate the HPLC/UV analysis of multiple oligo samples and utilize HPLC/CMS analysis to determine their molecular weight.
In this application note, we use whey protein isolate and two casein macro peptides as an example to demonstrate the isolation and purification of bioactive peptide components with a system comprised of a puriFlash® 5.250 prepLC coupled online to an expression® CMS detector.
In this application note, we demonstrate a complete solution for the synthesis, purification, and quantitation of Gd(III)-DOTA, an ionic imaging contrast agent with better chemical stability and lower toxicity compared to the currently used Gd-(DTPA).
Iohexol is a widely used non-ionic imaging agent that improves contrast for x–ray analysis. Its low osmolality allows for a rapid clearance via the kidney, preventing reabsorption and further metabolization. This makes iohexol a compound with a better safety profile compared to other imaging agents.
In this application note, a simple and accurate HPLC-CMS method for iohexol analysis is introduced.
In this application note, we show how Cyanidin-3-glucoside can be extracted from black rice and purified with a prepLC/Flash system. The amount and purity of Cyanidin-3-glucoside was measured utilizing a certified reference standard.
In this application note, a method to separate and purify green tea catechins with preparative liquid chromatography (Prep-LC) using the puriFlash® 5.250 system is demonstrated. An additional HPLC-UV-MS method is also demonstrated for compound confirmation and purity analysis.
In this application note, a simple, cost-effective UHPLC/UV method is introduced for analysis of cannabinoids in edible samples (examples shown are gummy bears, chocolates and cookies) using the Advion Interchim Scientific AVANT™ UHPLC/PDA System.
In this application note, a simple, cost-effective UHPLC/UV method is presented for the quantitation of cannabinoids using an Advion Interchim Scientific AVANT™ UHPLC system. With simple sample preparation, requiring only dilution, the method can be used for the analysis of plant material, oils, distillates and isolates.
In this application note, both the purification and analytical processes for THC remediation using the Interchim ITM-5015 and Advion AVANT™ HPLC-UV systems are shown to form a complete solution for THC remediation in the hemp industry.
