Mass spectrometry guided purification for efficient isolation of natural products at semi- and preparative scale

In natural product research the isolation of compounds from crude extracts is a key element. An increase in the efficiency of the purification process, by improvement of instrumental and methodological approaches, remains a crucial point. In this respect, modern flash chromatography, has evolved and uses 15 μm spherical particles for efficient separation at moderate pressures. Flash C-18 was tested for the rapid isolation of various plant secondary metabolites of interest.