The expression CMS provides essential compound information quickly and improves the chemist’s workflow. The Atmospheric Solids Analysis Probe (ASAP®) permits fast analysis of solid and liquid samples and is a simple, faster and lower cost alternative to LC/MS methods. This direct analysis probe method has been shown to be useful for the analysis of volatile and semivolatile compounds. ASAP is available as a combined ASAP-APCI source, or existing APCI sources can be easily modified to become dual APCI-ASAP capable.
Register to download the full application note which includes the results of an example analysis.
Q: What is the focus of your lab’s research?
A: Reach Separations is a contract research organization that services chemical, agricultural, pharmaceutical and academic clients.
Capability developed around expertise in separation science, specifically purification/prep.
Q: What previous workflow challenges did you experience?
A: Needed a tool to confirm quality of material (in and out).
Q: Why did you incorporate the expression CMS into your laboratory?
A: We selected the expression CMS for several reasons; proper MS Detector at an attractive price as well as its compact size because our lab space is limited. Advion’s technical support and service is exceptional. Easy to integrate with our wide range of equipment. Vendor relationship that is two way, Advion listens to our needs and ideas.
Q: Who Would you Recommend to purchase the expression CMS?
A: Anyone who needs a reliable mass spectrometer upgrade/addition for existing equipment.
Q: WHat is tHe focuS of your lab’S reSearcH?
A: The group has comprehensive experiences for undertaking radiotracer experiments, different spectroscopic techniques, characterization of colloid-chemical properties and in vitro/in vivo studies in view of the development and characterization of novel radiotracers, including functionalized nanoparticles. The design and development of chemically, metabolically and radiolytically stable radiometal complexes on the basis of 64Cu/67Cu is of special interest. Furthermore, polynuclear metal compounds (polyoxometalates, Re clusters) for anti-tumor activity, photosensitizing, and radiation-sensitizing properties are under investigation. The characterization of binding and transport behavior of novel receptors by solvent extraction studies represents one main topic.
Q: WHAT PREVIOUS WORKFLOW CHALLENGES DID YOU EXPERIENCE?
A: The development of new bifunctional chelating agents and relevant target-seeking bio-conjugates requires novel synthetic strategies and the improvement of known pathways as well. For the successful achievement of this goal, ‘real-time’ characterization of main and side-products is of particular importance.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: The Advion expression compact mass spectrometer rapidly provides reliable mass data in a stand-alone modus and coupled to UPLC/HPLC systems, respectively. Sample handling and measurement are straightforward. PhD students appreciate both the ‘in-time’ information about success and failure in developing novel compounds. I recommend to all groups requiring immediate access to reliable mass data for the characterization of novel compounds.
Q: What is the focus of your lab’s research?
A: The mass spectrometry facility here at Boston College’s main focus is as a core lab, open to all users, both undergraduate and graduate students alike.
Our main research interests include the application of mass spectrometry as a routine tool for chemistry/chemical biology; exploring new research applications for mass spectrometry; and the use of open-air ionization methods for the analysis of pharmaceutical, biological, drug and chemical analysis.
Q: What was your previous work flow or challenges?
A: As a core lab we have a large number of samples being run and there is a constant need to change ionization sources.
Q: Why did you incorporate the expression® CMS into your laboratory?
A: The expression® has allowed us to take the workload of the other more expensive instruments by incorporating it as an open access instrument. With minimal training, the students are able to run their own samples, as not to slow down their research.
Due to the instrument size and speed at which it pumps down, we are able to move the instrument directly to the classroom for students to use.
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: The central activity of our group is to design and develop molecules to study and control biological systems. We are using organic and organometallic synthesis to construct new bioactive compounds, new agents for bioconjugation, and new probes for biology. Our focus is to apply chemistry to biology by identifying targets and then creating cancer treatment strategies and therapy options.
Q: WHAT PREVIOUS WORKFLOW CHALLENGES DID YOU EXPERIENCE?
A: For identification and analysis, we use NMR and HPLC regularly, but had no mass spectrometer in the laboratory, so we gave our samples to outside MS service for analysis. Therefore, we had to wait for the results.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: Advion’s CMS allows us to have a self-service option. One ingenieur is in-charge of the CMS, but everyone can use it including students after a short formation. And for 80% of our work it is perfect. We still use the MS facility for more demanding analysis, but this new workflow speeds up our process significantly.
Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE EXPRESSION CMS?
A: I would recommend the CMS to any small or large organization like ours who wants fast answers for day-to-day analysis.
Q: What is the focus of your labs research?
A: Our main research interest is to use mass spectrometry as a routine tool for synthetic intermediates.
Q: What previous workflow challenges did you experience?
A: As we have large numbers of products to run, our major workflow issue is to shorten product detection times.
Q: Why did you incorporate the expression® CMS into your lab?
A: As an open-access instrument, the expression® CMS allows us to incorporate with other instruments with a compact size. More important, it helps us to know the molecular weight information of products timely.
The purpose of this study was to evaluate the positive and negative electrospray (ESI) and atmospheric pressure chemical ionization (APCI) LC/MS ion current response from a representative synthetic mixture of standard pharmaceutical compounds. The selected drup compounds were prepared as an equimolar mixture and analyzed by an optimized gradient LC/MS procedure using a novel compact mass spectrometer. The total and extracted ion current chromatographic peaks were compared between ESI and APCI in both positive and negative ionization modes. The results from this study shed some light on the relative merits of APCI vs ESI as well as the importance of the optimal ionization polarity for the LC/MS determination of the selected acidic and basic small molecule drugs. We also note some differences in adduct formation between these two API techniques.
Q: What is the focus of your lab’s research?
A: To address unresolved questions in DNA chemical biology, our group develops molecular probes for characterizing the structure, function, and dynamics of nucleic acids in vivo. Daily work in our lab relies heavily upon the rational design and synthesis of new organic compounds and their metal-containing complexes.
Q: What previous workflow challenges did you experience?
A: One major challenge to our workflow is the rapid identification of new target molecules that are often prepared as complex mixtures.
Q: Why did you incorporate the expression CMS into your laboratory?
A: We chose the expression CMS as a rapid and sensitive screening tool to identify new target molecules from the chromatographic separation of crude reaction mixtures. Given the exceptionally high sensitivity of this system, desired compounds that are initially made in trace quantities can be easily identified.
Q: What is the focus of your lab’s research?
A: Medicinal chemistry research in my lab is a collaborative exercise where we engage other scientists with complementary interests. Currently, we are engaged in the discovery of protein kinase inhibitors for anti-infective and anti-inflammatory applications and in the discovery of new agents useful for the potential treatment of neurodegenerative diseases. We are working with others at MSU as well as scientists in other universities and research institutes.
Q: Why did you incorporate the expression CMS into your laboratory?
A: As a medicinal chemist working in the industry, I wanted to have access to the same technology for my group’s research at MSU. However, working in the industry I had experts to run samples. In my lab at MSU, it is essential to have the same technology available in an easy to use the instrument. Not only do the expression CMS specifications meet our needs perfectly, but also it runs without a hitch and it is easily serviceable by a non-expert.
Q: Who would you recommend to purchase the expression CMS?
A: I would recommend the expression CMS to any non-expert chemist or laboratory who needs access to this level of technology. The Advion service and support groups make it accessible.
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: The focus of the team is to develop innovative products and applications for thin layer chromatography (TLC). Intensive research and development is done to couple TLC with mass spectrometry (TLC/CMS), a field of growing interest.
Q: WHAT PREVIOUS WORKFLOW CHALLENGES DID YOU EXPERIENCE?
A: Our major workflow issue is to shorten product development times and to deliver our customer valuable applications for new products. The CMS in combination with the elution based TLC/MS interface supports us reaching these goals.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: The expression CMS is a compact, robust and easy-to-use instrument. Together with the elution based TLC/MS interface, an affordable complete system is on the market, providing a great opportunity that can advance the TLC/CMS technology.
