USDA-ARS, Cornell University, Michigan State University
Abstract
The common dry bean (Phaseolus vulgaris L.) is a globally produced pulse crop and an important source of micronutrients for millions of people across Latin America and Africa. Many of the preferred black and red seed types in these regions have seed coat polyphenols that inhibit the absorption of iron. Yellow beans are distinct from other market classes because they accumulate the antioxidant kaempferol 3-glucoside in their seed coats. Due to their fast cooking tendencies, yellow beans are often marketed at premium prices in the same geographical regions where dietary iron deficiency is a major health concern. Hence, this study compared the iron bioavailability of three faster cooking yellow beans with contrasting seed coat colors from Africa (Manteca, Amarillo, and Njano) to slower cooking white and red kidney commercial varieties. Iron status and iron bioavailability was assessed by the capacity of a bean based diet to generate and maintain total body hemoglobin iron (Hb-Fe) during a 6 week in vivo (Gallus gallus) feeding trial. Over the course of the experiment, animals fed yellow bean diets had significantly (p ≤ 0.05) higher Hb-Fe than animals fed the white or red kidney bean diet. This study shows that the Manteca yellow bean possess a rare combination of biochemical traits that result in faster cooking times and improved iron bioavailability. The Manteca yellow bean is worthy of germplasm enhancement to address iron deficiency in regions where beans are consumed as a dietary staple.
Analysis was performed by LC/MS using the Advion expression Compact Mass Spectrometer (CMS).
The cannabinoid makeup of cannabis has significantly changed over the past few decades. Efforts to increase the psychotropic effects of cannabis have resulted in a THC content for current strains of the crop of 30 to 40 percent compared with only 5 percent 30 years ago. Up-regulating the THC content of the plant causes a loss of other cannabinoids the plant is producing. Some of these other cannabinoids, such as CBD, are believed to be responsible for its medical benefits.
Furthermore, no pesticides can currently legally be used during the production process. However, a recent test showed that two out of three samples of legal cannabis samples had pesticide residues above the legal limit for an edible. Once sample exceeded the limit by a factor of 1,600. Such a sample poses a consumer health risk and clearly shows the potential for illegal growing procedures or unclear product streams in the marketplace.
Additionally, cannabis contains more than 400 chemical compounds, 80 of which are unique to cannabis. This fact, plus the finding of cannabinoid receptors in human nerve, immune and brain cells explains why this product is such an interesting target for medicinal use.
This webinar examines three different workflows surrounding compact mass spectrometry as they apply to cannabis law enforcement, it’s natural product research, and product control.
In this webinar, Advion’s Scientific Founder, Jack Henion, Ph.D., will discuss his research using a direct analysis probe coupled with the expression CMS to analyze chemical compounds in Cannabis.
During this webinar you will learn more about:
A simple, sensitive and selective ASAP sample introduction approach to measure the presence and relative quantitative composition of two isobaric compounds, CBDA and THCA, contained in a complex sample such as hemp or cannabis plants or their corresponding extraction products.
Measurement of differences in the relative composition of CBDA and THCA fragment ions originating from the same precursor ion.
Applicability to screening plant and plant product materials such as hemp or marijuana to monitor out-of-specification composition.This webinar was featured by Lab Manager’s Tech Trends webinar series, “Tools for the Cannabis Testing Lab.”
This webinar was hosted by Lab Manager and recorded 4/11/19.
Volatile Atmospheric Pressure Chemical Ionization (vAPCI) combined with Advion Interchim Scientific’s expression® compact mass spectrometer (CMS) is a fast and easy method to analyze the headspace of a sample with no additional sample prep.
The vAPCI ion source is a simple, powerful tool for gas phase sample analysis. The technique can quickly and confidently identify compounds in air, breath, headspace and other gas samples.
Direct sample analysis of liquids, solids and powders without chromatography is as easy as it sounds, and provides mass spectral information within seconds. The video demonstrates analysis of a reaction mixture.
Advion Interchim Scientific’s Plate Express® is ideal for fast, easy TLC/MS analysis. Coupled with the expression® Compact Mass Spectrometer (CMS), the TLC plate reader allows prep-free sampling and data in less than 30 seconds.
Advion’s direct atmospheric solids analysis probe, otherwise known as ASAP®, permits chemists fast and reliable sample analysis of liquid and solid samples such as reaction mixtures, food samples, natural products and tablets.
Advion’s expression® CMS paired with the Atmospheric Solids Analysis Probe (ASAP®) can quickly and easily be used to screen an unidentified drug mixture in this mobile field testing dramatization.
We demonstrate a cost-effective UHPLC/CMS method for the analysis of chloramphenicol in milk with a detailed discussion of sensitivity, linear response range, carryover and robustness using the Advion expression® Compact Mass Spectrometer (CMS) and Advion AVANT™ UHPLC System.
This application note was adapted from a poster that was presented at the 2019 ASMS Conference on Mass Spectrometry and Allied Topics in Atlanta, GA.
Using separation from the AVANT™ UHPLC system coupled to the expression® Compact Mass Spectrometer (CMS), cannabinoids from commercially available CBD oils are measured for concentration against product label claims for a comparative test.
This poster was presented at the 2019 Cannabis Science Conference East in Baltimore, MD.
