In this webinar, Heather Walker from Sheffield University’s plant production and protection team explains how taking a compact mass spectrometer from the lab to the field helped optimise the team’s research.
The webinar highlights the advantages of mobile mass spectrometry, and introduces a mobile mass spec vehicle that has been outfitted to optimize time in the field without sacrificing results for real-time decision making about sampling.
During this webinar you will learn:
about fast and easy sample techniques available with compact mass spectrometry, including direct probe for liquids and solids, and a volatile headspace analysis tool – both with no sample preparation required
how instant data can be obtained to provide real-time decision making, allowing for smaller sample sizes to optimise reproducibility – without the need to oversample with the intent of bringing samples for off-site lab testing
how the metabolic profile of wheat can be measured via compact mass spectrometry, highlighting the success of the technology in the food and ingredients testing industry
This webinar is brought in association with Advion and will feature presentations from Advion’s scientific founder Jack Henion and Sheffield University’s Heather Walker.
Integrated Solutions for Food Authenticity, Characterization and Safety with the expression® Compact Mass Spectrometer, AVANT® (U)HPLC and SOLATION® ICP-MS
Your integrated laboratory solution for comprehensive testing of food, ingredients and natural products. Advion offers a full suite of analytical instrumentation for characterization, screening and purity determination, including:
expression® CMS – The expression® Compact Mass Spectrometer (CMS) offers the industry’s widest range of sample introduction systems, providing rapid answers with little or no sample prep in < 30 seconds.
SOLATION® ICP-MS – Provides fast, simple, multi-element analysis of toxic metals, and essential elements and minerals in a wide array of samples, including water, soil, plant material, food and raw ingredients
AVANT® HPLC & UHPLC – Advion’s range of modular high performance, liquid chromatography systems can be used standalone with UV and UV/Vis detector options, or with the expression® CMS to provide seamlessly integrated LC/CMS under the full control of Advion’s simple, intuitive software suite.
PLATE EXPRESS® TLC PLATE READER – Plate Express® provides a simple, automated means of obtaining mass spectra directly from TLC plates, combined with Advion’s expression® CMS creating a technique known as TLC/ CMS. See results in < 30 seconds at the push of a button.
For Direct Mass Analysis of Liquid & Solid Samples with the expression® CMS
The direct atmospheric solids analysis probe, otherwise known as ASAP®, permits chemists fast and reliable sample analysis of liquid and solid samples such as reaction mixtures, food samples, natural products and tablets.
Chemists simply dip the closed end of a glass capillary into the sample of interest (even complex mixtures) or scrape the surface of a solid, and then place the capillary into the CMS for analysis.
Clean-up and analysis is a single step. Involatile buffers and salts remain on the capillary ensuring they do not suppress ionization or interfere with the measurement, while the compounds of interest are identified by the CMS.
In addition, users benefit from solvent-free APCI, which ionizes a wider range of compounds.
Provides simple, automated MS analysis in seconds, directly from TLC plates
The Plate Express® is a device that provides a simple, automated means of obtaining mass spectra directly from TLC plates, creating a technique known as TLC/MS. Combined with Advion Interchim Scientific’s expression® compact mass spectrometer (CMS) this becomes TLC/CMS. Using this technique, chemists can quickly and confidently identify products even in complex mixtures without additional sample preparation.
Identify spots in <1 minute
Avoid the risk of overloading the mass spectrometer – TLC spots contain the ideal amount of sample for mass spectrometry
Software controlled – spectra obtained within a few mouse clicks
Simplify the process of obtaining spectra – ideal for multi-user labs
Providing simple, high-performance, LC/CMS with the expression® CMS
Modular, stackable design, with many options provides custom solutions for both HPLC and UHPLC needs. From the simplest manual injection HPLC to a fully automated, streamlined UHPLC system and everything in-between, the Avant series can be configured to fit your analytical requirements and your budget.
The AVANT® (U)HPLC series is the latest in Advion’s innovative line of sample inlet devices for the expression® CMS which include:
Plate Express® for automated mass assays from TLC plates
The ASAP® direct analysis probe for solid, liquid and even air sensitive samples using iASAP
vAPCI for analysis of volatile organic compounds (VOCs) from head spaces, breath, and other gas samples.
Flow chemistry interfaces
All these techniques can be simply, simultaneously, interfaced with the CMS so that the chemist can move quickly and effortlessly between workflows.
For One-Touch Mass Analysis of Solids, Liquids,Surfaces and Fibers with the expression® CMS
The Touch Express™ Open Port Sampling Interface (OPSI), is designed for simple sampling of surfaces, solids, liquids and sample preparation tips and fibers. The novel ambient sampling technique was developed by Gary Van Berkel and Vilmos Kertesz, of Oak Ridge National Laboratory.
Paired with the electrospray ion source of the expression® Compact Mass Spectrometer, the solvent forms a meniscus at the open port before being drawn down the inner path into the electrospray ion source of the mass spectrometer under the Venturi effect of the nebulization gas. Any soluble sample touching the port is analyzed by the mass spectrometer in just seconds. Touch Express™ OPSI offers a fast assay bench-top solution for solid, liquids, and surfaces in a small-footprint, easy-to-use system.
There are many separation and detection methods that are available for the determination of saccharides. These include GC-MS with derivatization, HPLC-UV with derivatization, HPAEC-PAD (High-performance anion-exchange chromatography coupled with pulsed amperometric detection), and LC-MS. The availability of a sensitive and accurate method is still a challenge. For an LC-MS method, given the high polarity, hydrophilicity and low proton affinity of these compounds, it can be difficult to ionize them directly by electrospray by ESI or APCI. The chemical diversity and numerous isomers of carbohydrates further complicates their analysis with LC-MS.
In this Application Note, post-column chloride attachment is used for the analysis of samples containing carbohydrates using the Advion expression® Compact Mass spectrometer (CMS). The 35Cl and 37Cl isotopes also provide confirmation for the formation of the chloride adducts in negative ion APCI mode. Four carbohydrates (Fructose, Glucose, Sucrose and Lactose) are used for method verification.
Introducing the new SOLATION® Inductively Coupled Plasma Mass Spectrometer (ICP-MS) – the ultimate instrument for multi-element analysis, the SOLATION® provides high sensitivity measurement of trace elements from a wide range of matrices including complex samples such as urine, serum, plasma, whole blood and tissue samples. Designed for ease of use, with intuitive software, the SOLATION® ICP-MS is the ideal instrument for environmental, pharmaceutical, food safety and clinical laboratories everywhere.
Fill out the form to download the full SOLATION® ICP-MS brochure now.
The Touch Express™ Open Port Sampling Interface (OPSI), is designed for simple sampling of solids, liquids and sample preparation tips and fibers. The novel ambient sampling technique was developed by Gary Van Berkel and Vilmos Kertesz, of Oak Ridge National Laboratory.
Paired with the electrospray ion source of the expression® Compact Mass Spectrometer, the product incorporates a low volume, open port of continuously swept solvent, flowing directly into the electrospray ion source of the mass spectrometer.
During this webinar you will:
Learn how any soluble material touching the port is analyzed by the expression® Compact Mass Spectrometer in just seconds.
Learn how the sample technique eliminates sample preparation and provides zero carry over.
Find fast analysis methods for solids, liquids and sample preparation tips and fibers.
M.R. Chae, S.J. Kang, K.P. Lee, B.R. Choi, H.K. Kim, J.K. Park, C.Y. Kim, S.W. Lee
Onion (Allium cepa L.) and quercetin protect against oxidative damage and have positive effects on multiple functional parameters of spermatozoa, including viability and motility. However, the associated underlying mechanisms of action have not yet been identified. The aim of this study was to investigate the effect of onion peel extract (OPE) on voltage-gated proton (Hv1) channels, which play a critical role in rapid proton extrusion. This process underlies a wide range of physiological processes, particularly male fertility. The whole-cell patch-clamp technique was used to record the changes in Hv1 currents in HEK293 cells transiently transfected with human Hv1 (HVCN1). The effects of OPE on human sperm motility were also analyzed. OPE significantly activated the outward-rectifying proton currents in a concentration-dependent manner, with an EC50 value of 30 μg/mL. This effect was largely reversible upon washout. Moreover, OPE induced an increase in the proton current amplitude and decreased the time constant of activation at 0 mV from 4.9 ± 1.7 to 0.6 ± 0.1 sec (n = 6). In the presence of OPE, the half-activation voltage (V1/2 ) shifted in the negative direction, from 20.1 ± 5.8 to 5.2 ± 8.7 mV (n = 6), but the slope was not significantly altered. The OPE-induced current was profoundly inhibited by 10 μm Zn2+ , the most potent Hv1 channel inhibitor, and was also inhibited by treatment with GF109203X, a specific protein kinase C (PKC) inhibitor. Furthermore, sperm motility was significantly increased in the OPE-treated groups. OPE exhibits protective effects on sperm motility, at least partially via regulation of the proton channel. Moreover, similar effects were exerted by quercetin, the major flavonoid in OPE. These results suggest OPE, which is rich in the potent Hv1 channel activator quercetin, as a possible new candidate treatment for human infertility.
The LC/MS analysis was carried out using Advion Expression® CMS.
