Application Note

Recommendations for Transferring Peptide Mapping Methods to LC/MS Using Core-Shell Columns

With the emergence of new protein therapeutics, including biosimilars, biobetters and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation.

The purpose of this study is to provide guidelines for developing optimal LC running conditions with Aeris™ PEPTIDE core-shell HPLC/UHPLC columns and determine the  feasibility of transferring a method developed on UV to MS simply by adjusting the acidic modifier in the mobile phase.

To download, please fill out the form below.

Advion respects your privacy. We will not give your email address to third parties or send you unwanted email.