HRMS, Chemical Modification and Liquid Extraction Surface Analysis for Lipidome Profiling of Colon Adenocarcinom

Presented by: Gavin Reid, Associate Professor at Michigan State University

A large number of studies have demonstrated that disruption of lipid metabolism or signaling pathways can play a key role in the onset and progression of human disease, including cancer and diabetes. Thus, a comparative analysis of changes in individual lipids or lipid profiles (i.e., the lipidome) between normal and diseased cells, tissues, organs, or accessible bodily fluids (e.g., tumor interstitial fluid, blood plasma or serum), may enable the identification and characterization of lipids that can serve as effective biomarker signatures of the disease. In this presentation, the development and application of a straightforward and high throughout analysis strategy consisting of high-resolution ‘shotgun’ mass spectrometry (MS), ‘targeted’ tandem mass spectrometry (MS/MS), functional group specific chemical modification and in situ liquid extraction of cell culture samples is described for the comprehensive identification, characterization and quantification of multiple lipid classes from within a colon adenocarcinoma cell line, SW480, and its metastasized derivative, SW620.