TriVersa NanoMate®
Nanoelectrospray ionization technology
The TriVersa NanoMate LESA® is the latest in chip-based electrospray ionization technology from Advion Interchim Scientific®. It combines the benefits of liquid chromatography, mass spectrometry, chip-based infusion, fraction collection, and direct surface analysis into one integrated ion source platform.
Obtain more information from complex samples than LC/MS alone
The TriVersa NanoMate® integrates with:
- Advion Interchim Scientific® ESI Chip®
- LC/MS with Fraction Collection for Re-Analysis by Infusion
- Liquid Extraction Surface Analysis (LESA® and LESAplus)
Advion Interchim Scientific® ESI Chip®
The ESI Chip® unleashes the power of mass spectrometry through miniaturization. This microfluidics chip contains an array of 400 nano-electrospray nozzles, etched in a silicon wafer. The fabrication method is highly reproducible to ensure the integrity and equal performance of each nozzle. The electrical field created by the nano-electrospray nozzles results in a highly efficient ionization and long-lasting, stable spray from µL of sample volume.
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- ESI chips are available in three different nozzle emitter sizes from 2.5 µm to 5.5 µm ID
- Internal chip void volume in the pL range allows for 15 min spray times from only 3 µL of sample
- Automated sample handling can deliver spray from samples in 96 or 384 well plates
- Silicon chip design delivers superior electric field strength and ionization efficiency
- Internal counter electrode allows for spray sensing and recovery feature
Chip-based Direct Infusion and LC/MS Fraction Collection with Re-analysis by Infusion
The TriVersa NanoMate® is the only automated ion source platform that allows high throughput infusion experiments for small or large analytes in applications ranging from shotgun lipidomics and non-covalent interaction studies (NCI) to protein characterization.
The TriVersa NanoMate® was developed to operate as a nano-ESI ion source for LC/MS applications. Excess LC eluate can be utilized for additional fraction collection to create physical storage of the LC/MS experiment and allows re-analysis of fractions of interest as identified by the online LC/MS run.
Liquid Extraction Surface Analysis (LESA® and LESAplus)
LESA was developed in collaboration with Dr. Gary van Berkel from the Oak Ridge National Laboratories. Its unique surface extraction via a liquid droplet held in suspension (liquid junction) allows a novel approach for spatially resolved analysis of biological and technical surfaces of interest:
- Fast, simple and direct analysis
- 1 mm spatial resolution (LESA); 400 µm resolution (LESAplus)
- Liquid extraction and nESI based analysis
- High sensitivity due to nESI ionization and analyte extraction into only 1 µL of solvent
- Optional nLC separation (LESAplus)
