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Better Chemistry from Intelligent Flash Purification

<< Click here to check out our latest whitepaper:
Breaking Through Bottlenecks in Organic Synthesis with a Streamlined Purification Workflow >>

We have all been there: You want to do a simple Flash purification.

Easy, right?

You then begin to prepare your reaction mixture, think about monitoring the reaction, confirming the product, developing the Flash method…

This post highlights some ways to make Flash purification easier than ever before. Read on for tips on reaction monitoring, purification and fraction ID.

<<click infographic to zoom>>

Pre-Flash Run: Performing (and monitoring) your reaction
Starting off you are faced with several decisions to make – decisions that can turn the simple purification process in to a half-day adventure in sample preparation and scrolling through social media while you wait for your LC/MS results.

The first decision you need to make is how you can quickly and easily make your compound and get that compound over to the Flash system. Pronto!

Tip #1: Reduce wait times.
If you think, “LC/MS is best!” you may want to reconsider. Reaction monitoring can be done much faster and easier using alternative technologies like TLC. Consider what you truly need – a quick answer to, ‘Did I make my compound?” Seek to find that answer in the easiest way possible, using the most straightforward (even basic) technology that you have on hand in your lab.

Tip #2: Reduce your sample preparation.
If you choose to use the TLC route over LC/MS (Bravo!) to monitor the reaction, you’ll still have a few steps between you and the Flash run. Namely, the annoyance of scraping spots, preparing them in a solvent for direct injection in to the mass spectrometer. It may be an opportunity to explore additional technologies available on the market, such as a TLC Plate Reader. This newer technology gives you push-button analysis of TLC plates. No scraping. No sample prep. No cleanup. Just results in seconds.

Imagine – your reaction complete and your compound of interest identified with only the push of a button!

<<click here for more information on reaction monitoring from Advion>>


During the Flash Run: Use the Tools at Hand
The nice thing is that (generally) technology is on our side. Thanks to AI and auto-runs and the ‘set-it-and-forget-it’ mentality of several tedious chemistry processes, we have luckily evolved to a time where many industry-leading experts have done the work for you.

Tip #3: Simplify Flash method development.
Several questions come up during method development in Flash that, unless you are an expert, you may not know definitively. What are your Rf values? Which column should you choose? If your Flash system offers the ability to simplify method development with suggested details, it is a good starting point and can help your routine purification run smoothly.

<<click here for more information on intelligent flash purification from Advion x interchim>>


After the Flash Run: Identifying Fractions
You’ve made it though your purification and feel confident about the work you put in so far. Hopefully you spent no time at all monitoring your reaction and preparing your method, and you feel confident that you let the Flash system help with method development. But what now? Is it finally time to dust off the old LC/MS?

Tip #4: Simplify Fraction ID
There is not one way to ID a compound. As we learned before, TLC is an ideal alternative to LC/MS for reaction monitoring. Now, we look at it from a different angle – how to quickly ID the fractions in your tubes without wasting any of your precious time?

Tools like the Advion ASAP liquids and solids probe are ideal fo this. The simple probe is dipped in to one of the tubes and then inserted in to the APCI ion source of the mass spec. This allows for prep-free sampling – and another way to skip the LC/MS.

<<click here for more information about the ASAP probe from Advion>>

Have any tips (or headaches) to add to this list? Email us, info@advion.com and make yourself known! We are happy to connect you with a Flash expert on our team.

Interested in a streamlined TLC to Flash to ASAP workflow system? Advion x Interchim is currently offering a free Flash system with the purchase of a mass spectrometer. Click here to learn more about the latest special offers.

Video more your style? Watch a full Flash workflow, from reaction to fraction in our latest clip:

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Advion, Inc.’s parent company, Bohui Innovation Biotechnology, will acquire the Interchim group and subsequently combine with Advion. Global commercial combination and impact should be notable.

BEIJING, CHINA, ITHACA, NY, USA and MONTLUÇON, FRANCE, February 25, 2020 /PRNewswire/ — Advion Inc. today announces parent company Beijing Bohui Innovation Biotechnology Co., Ltd., has filed its intention to acquire Adchim SAS, the parent company of the Interchim group companies. This transaction will subsequently result in the combination of Bohui’s subsidiary Advion Inc., a leader in high performance, small footprint mass spectrometry and Interchim SA, a global provider of chromatography and purification instrumentation and consumables for life sciences, research and industry. Building upon seven years of a successful OEM partnership, the companies’ future elevated multi-national combination for sales of instruments, consumables and customer service is expected to rapidly grow revenue.  The combined suite of solutions will be displayed at global conferences beginning in early March, though each company will sustain direct customer transaction relationships until the subsequent combination.

The boards of both companies have agreed to the transaction, which remains contingent upon regulatory approvals and other customary matters.

Complementing Advion’s strong academic, pharma, biopharmaceutical and clinical position in mass spectrometry and chromatography products, Interchim adds a broad range of flash chromatography, preparative LC systems, SPE, evaporation, chromatography columns and consumables to the company’s combined product portfolio. Partners since 2012, Advion and Interchim led the market by being the first to develop integrated Flash-MS and seamlessly coupling Advion’s high performance expression® Compact Mass Spectrometer with Interchim’s puriFlash® chromatography systems. In the future, the combined companies will strengthen the integration and breadth of workflow solutions by adding Advion’s proprietary range of novel sample introduction and assay techniques.

In addition to enhanced product integration, the future combination will offer a single source for all instrumentation, column chromatography, sample prep, and consumables sales. Coupled with complementary geographical and market segment strengths, the eventual combination will drive synergistic growth of both companies.

“We are thrilled to combine with Interchim,” said David B. Patteson, who will lead the forthcoming combined Bohui Instrument Group. “Our expanded solution deck and global scale will provide a formidable platform for growth – offering novel media, columns, prep LC, HPLC, flash chromatography, mass spectrometry, sample prep, and much more. Our customers will benefit from an expanded workflow solution portfolio. Interchim’s exceedingly strong Central EU market share and presence, coupled with their dynamic chromatography column business is a perfect commercial fit with Advion’s LC/CMS offering.  Together with Interchim, we have built strong relationships which persist to this day.”

“We are really excited about the perspective of this strategic combination,” said Lionel Boch, Président du Directoire Interchim. “As a guarantee of a promising future, it represents today for Interchim, its employees, suppliers and partners a perfect fit. The strengths of the two entities, already working together successfully, will be enhanced by this new step and will enable us to reach and develop unparalleled scientific solutions and technological platforms for our customers around the world.”

Customers and industry partners will benefit from this increased solutions portfolio and can expect significant new developments in mass spectrometry, flash and preparative chromatography of small and large molecules over the course of 2020.

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About Advion, Inc.

Advion’s nearly three-decade dedication to serving scientists yields customer-focused life science solutions. Our deep scientific, engineering and customer workflow knowledge spawns an unrivaled solution portfolio.  We work directly with, train, and passionately advocate for our customers to ensure their success. Dedicated to Science – Dedicated to You. More about Advion, Inc. can be found on our website, www.advion.com.

About Interchim

Interchim SA is a French multinational company dedicated to Sciences with its own R&D laboratory. Founded in 1970, the company has 50 years of experience and knowledge in the field of synthesis, life sciences, as well as analysis and purification by LC chromatography. Our products and services are today available in 60 countries through  our direct sales, subsidiaries and distributors network. For more, visit www.interchim.comand www.flash-chromatography.com .

For Advion, Inc.

Kristy Licari
Director of Marketing
Advion, Inc.
Telephone: 1-607-882-9903
Email: klicari@advion.com

For Interchim
Alexandra Boch
Directeur general adjoint, Marketing and Digital
Interchim
Telephone: +33 4 70 03 88 55
Email: alexandra.boch@interchim.fr

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Mass Spectrometry for the Teaching Lab

Teaching Mass Spectrometry: The Basics

Developing a curriculum for any course is taxing: lecture content, lab materials, take-home packets and more.

Our team at Advion understands that while teaching mass spectrometry to your students may be a priority for you, it may not be something that you are ready to do without significant preparation. That’s why we have developed a teaching package, featuring the following:

  • The expression® Compact Mass Spectrometer (CMS) – an instrument with the simplicity for teaching students, and the performance to support your own research
  • ESI and APCI ionization techniques
  • Easy to use sample inlet techniques such as the Plate Express automated TLC plate reader, and the one-touch ASAP liquid and solids probe
  • Over 10 hours of recorded lectures from industry expert and Advion co-founder, Dr. Jack Henion
  • Lecture slides to share or adapt to your own teaching style
  • Simple, intuitive, student-friendly CheMS Software

From introductory mass spectrometry principles to in-depth research projects, Advion’s teaching package provides a wide array of hands-on experience opportunities essential for teaching mass spectrometry to chemists.

 

Learn more about the easy, prep-free sample introduction techniques that make learning mass spectrometry with the expression CMS stimulating and easy for both professors and students:

 

Plate Express Push-Button TLC Plate Reader

• Couple the Plate Express TLC plate reader to the expression CMS for push-button TLC plate analysis

• No scraping or sample preparation required

• Identify spots in <1 minute

 

 

ASAP Atmospheric Solids Analysis Probe

• Ideal for student use, the ASAP probe provides the easiest sample inlet technique for liquid and solid samples with no prep

• Insert the probe into the mass spectrometer for answers in seconds

 

 

LC/CMS – Learning the Basics of (U)HPLC

• Gain expertise in LC/MS by adding the Advion AVANT (U)HPLC system, or connect the expression CMS to your lab’s current LC using our integrated software options

 

 

CheMS Software for a simplified user experience

• Quickly select the workflow and type of compounds to be analyzed without needing to be an expert in mass spectrometry

• Effortless method and instrument setup for a range of sample introduction techniques

 

 

 

expression CMS

The expression CMS is a research-grade benchtop mass spectrometer designed with the chemist in mind. When class is not in session, the system is ideal to support research projects. With electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources and an extended mass range option for large molecules, the expression CMS is an easy-to-use versatile mass detector for a variety of research applications and in the teaching lab. Its ease-of-use, versatility, and one-click software make the expression CMS ideal for undergraduate and graduate teaching chemistry programs.

 

 

Comprehensive Lecture Materials

Developed by Cornell Professor Emeritus, Jack Henion, PhD, along with leading chemistry departments, a ready-to-teach curriculum on mass spectrometry is included in the teaching package. Share as is or adapt to your teaching style. The curriculum includes pre-recorded lectures and accompanying slides on the fundamentals of mass spectrometry, sample inlet techniques, and real-world examples used in the chemistry workplace for hands-on learning. Lectures include:

  • Introduction to Mass Spectrometry
  • Flow Injection Analysis, ESI and APCI Ionization Techniques
  • Direct Analysis Probe for Liquids and Solids (ASAP)
  • Plate Express TLC Plate Reader
  • Touch Express Open Port Sampling Interface (OPSI)
  • LC/MS Techniques
  • Other CMS Applications & Techniques
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Comprehensive Reaction Monitoring & Flow Chemistry Resources

“Did I make my compound?”

 

It’s a common phrase that may follow you around the lab daily. With time-sensitive reactions coupled with the time-sensitive need to confirm data, fast reaction monitoring is essential for bench chemists looking for the most streamlined daily workflow.

The expression Compact Mass Spectrometer (CMS) addresses the need of organic and synthetic chemists to understand the optimal time to quench a reaction mixture. They can obtain answers within minutes rather than hours or days. Additionally, multiple inlet techniques like the atmospheric samples analysis probe (ASAP), Plate Express TLC plate reader, direct injection and more provides the ultimate in flexibility for sample analysis without the need for complex sample preparation. In addition, the use of the expression CMS in flow chemistry applications is an ideal reaction monitoring tool.

Learn more about the essential tools for fast reaction monitoring in this comprehensive resource guide:

 

Whitepaper: Compact Mass Spectrometry – A Complete Reaction Monitoring Solution

• Learn different sample techniques and ionization methods  based on your compounds

• See how prep-free, 30 second analysis can benefit your lab

• Find out how an optimized flow chemistry tool for fast reaction monitoring can take some burden off high resolution instruments with long run times

 

 

Webinar: Self-Optimizing Flow Reactors, Chris Horbaczewskyj, University of Leeds

• Learn advantages and disadvantages of both batch and flow chemistry

• Find out how on-line analytics within continuous flow chemistry allow reactions to be monitored in real-time allowing immediate characterization and the ability to optimize

 

 

Application Note: Real-Time Reaction Monitoring of a Solution-Phase Peptide Synthesis using the Plate Express and expression TLC/CMS

• Read how a combination of thin-layer chromatography (TLC) and compact mass spectrometry (CMS) is a simple and quick way for chemists to overcome synthetic challenges and optimize chemical reactions

• Learn how peptides of pharmaceutical interest can be readily synthesized following a rapid, continuous solution-phase synthesis strategy

 

 

 

Application Note: Analysis of Two Reaction Products by Direct Analysis Probe on a Compact Mass Spectrometer

• Find out how, with one swipe, a liquid or solid can be introduced in to the mass spec for reaction monitoring results in seconds

• Read how the ASAP with the CMS is used in identification of the products of a reaction where two products are possible

 

 

 

Application Note: Suzuki Reaction Monitoring Using Compact Mass Spectrometry with TLC Interface

• Learn how anonline thin-layer chromatography TLC/CMS technique using the Advion expression compact mass spectrometer (CMS) and Plate Express™ interface tcan provide compound structural information without sample preparation after TLC separation

 

 

 

Publication: A Novel Internet-Based Reaction Monitoring, Control and Autonomous Self-Optimization Platform for Chemical Synthesis (Open Access)

• Learn how the expression CMS was integrated in to a new workflow developed by the University of Cambridge Ley Lab that offers a modular software system that enables researchers to monitor and control chemical reactions via the Internet, using any device from any location in the world

 

 

 

Publication: Controlling an organic synthesis robot with machine learning to search for new reactivity (Open Access)

• Using the Advion expression Compact Mass Spectrometer and developments in robotics and AI, learn how chemical reactions can be performed faster than they can be performed manually. Not only is speed a benefit, the development also can predict the reactivity of possible reagent combinations after conducting only a small number of experiments

 

 

 

BONUS SOFTWARE WEBINAR: New Developments in Mass Spectrometry – Peak Express

• Find out if you made your compound with a new type of mass spectrum: the Delta Spectrum (ΔS). The patented ΔS is able to look beyond chemical noise and automatically detect even the smallest peaks without knowing the m/z

• How to easily find adducts, dimers, fragments, side-reactions and other unexpected compounds at the click of a button, in real-time or with post-processing

• How to use Peak Express software to highlight minor components in complex mixtures and dirty matrices, and can give you the ability to control mass-directed purification without providing the compound mass

• How to easily acquire XIC-quality data while scanning the entire mass range, allowing Peak Express™ to tell you the m/z