Quantitative spatial analysis of the mouse brain lipidome by pressurized liquid extraction surface analysis (PLESA)

Reinaldo Almeida, Zane Berzina, Eva C. Arnspang, Jan Baumgart, Johannes Vogt, Robert Nitsch, and Christer S. Ejsing

Here we describe a novel surface sampling technique termed pressurized liquid extraction surface analysis (PLESA), which in combination with a dedicated high resolution shotgun lipidomics routine enables both quantification and in-depth structural characterization of molecular lipid species extracted directly from tissue sections.