University of Birmingham, Cooper Mass Spectrometry Group
Q: What is the focus of your lab’s research?
A: My primary research is focused on characterization of biomolecular and non-biomolecular structures utilizing FT-ICR and orbitrap mass spectrometry. We are particularly interested in electron-based fragmentation techniques, such as ECD and ETD.
Specifically, we want to develop mass spectrometry methods to identify, characterize and quantify peptides; to understand peptide fragmentation hence identify and characterize proteins; to understand an array of biological processes through the identification and structural elucidation of post-translationally modified proteins and peptides.
Q: Why did you incorporate the TriVersa NanoMate® into your laboratory?
A: We use the TriVersa NanoMate for direct infusion and coupling to LC. Advion’s chip technology has revolutionized nanospray as far as ease of use. The ESI Chip is robust and bypasses any problems with non-uniformity. It allows us to move simply to the next nozzle if there is an issue with spray. The spray sensing capability is very clever and necessary for our overnight runs. We also use the TriVersa for our direct surface analysis of dried blood spots.
Q: To whom would you recommend the TriVersa NanoMate for their research?
A: I would recommend the TriVersa NanoMate to anyone with a mass spectrometer who uses nanoelectrospray.