Polish Academy of Sciences
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: The group has extensive experience in host-guest chemistry. We are seeking novel supramolecular platforms/systems, in particular macrocyclic systems, which might find applications in the recognition of ionic and neutral guest molecules. The research consists of two main parts: synthesis of novel receptors and analysis of their supramolecular assemblies. The formation of supermolecules is monitored and analyzed by NMR, UV-vis and fluorescence spectrometry. X-ray analysis is used to determine structure of receptors and their complexes. We also work with dynamic combinatorial libraries (DCL) and occasionally synthesize isotopically labeled compounds.
Q: WHAT WAS YOUR PREVIOUS WORK FLOW OR CHALLENGES?
A: In the synthesis of molecules of complex structures we need fast and reliable feedback about the success of each synthetic step. For example, macrocyclisation reactions sometimes give very complex mixtures. In order to follow the reaction course and facilitate purification, we need a fast tool for the unambiguous confirmation in which TLC spot is the desired product. Also, distinguishing between [1+1] and [2+2] macrocycles is not possible by simple NMR measurements and for new dynamic combinatorial libraries any HPLC characterization method required validation by an LC-MS in the service laboratory which takes time.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: We needed a versatile tool to speed up our synthesis processes and facilitate purification protocols.
CMS is mainly applied with Plate Express™ for fast compound identification from TLC plates. Now the choice of synthetic protocol can be evaluated within minutes. The product unambiguously spotted on the TLC plate is much easier to isolate. In addition, whenever needed we connect CMS to our HPLC system to analyze complex mixtures and to validate the analytical protocols. Isotope composition of labeled compounds is also easy to follow using this system.
Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE EXPRESSION CMS?
A: We would recommend it to all groups dealing with synthesis of small molecules. The instant feedback about the reaction progress and aid in isolation of the product saves a lot of time. With the expression CMS, the group is more independent, using the system as a stand-alone unit, coupled to the TLC plate reader or LC-system when needed.