Institute for Research in Biomedicine (IRB Barcelona), Spain
Q: What is the focus of your lab’s research?
A: Our goal is to provide the research community at IRB Barcelona and their co-workers with state-of-the-art tools and methodologies for the MS analysis of a broad range of biological species, from large proteins and DNA to small molecules. The final purpose is to get insight into these molecules’ identity, structure, interaction with other molecules and biological function in order to help in drug design, protein mechanism elucidation and in the search for biomarkers. We have implemented methods specialized in top-dwon proteomics and we are pioneers in this MS strategy in Spain.
As a core facility, we are responsible for working with different biologic molecules, and we are required to change methods constantly and efficiently.
Q: How does the TriVersa NanoMate® (TVNM) align with your research goals?
A: Originally, we purchased the TVNM for its chip-based direct infusion mode for noncovalent interaction analysis, but we learned quickly that it could be applied to other areas of our research. Prior to using the TVNM, the steps involved in collecting fractions were painful and time-consuming. With the TVNM, we can run LC/fraction collection or infusion without changing the setup and wasting time with stabilization. We do not experience the problems typical with traditional nanoelectrospray sources.
One aspect of the TVNM that impressed us was the ability to analyze complicated top-down samples with the LC compatibility. It is not possible to analyze these samples on an LC time scale, and the fraction collection capability allowed us to analyze in a way that was not possible previously.
Q: To whom would you recommend the TriVersa NanoMate for their research?
A: We use the TriVersa NanoMate for everything; noncovalent interactions, top-down, middle-down, bottom-up, basic infusion, LC coupled to fraction collection. The instrument is useful in all of its different set-ups, especially without having to change sources and waiting for a stable spray.
The reliability of the system is one of the greatest benefits especially for people who have to change frequently between applications. We have found the spray sensing feature to be very valuable because we know our precious samples will not be lost.