Real Time Reaction Monitoring of a Solution Phase Peptide Synthesis
Real-time reaction monitoring based on the expression compact mass spectrometer (CMS) can support the synthetic chemist to overcome synthesis challenges and optimize the reaction time on-line. Peptides of pharmaceutical interest can be readily synthesized following a rapid, continuous solution-phase synthesis strategy based on Fmoc protected amino acid building blocks 1. A simple model for such a reaction is the growing of analogues of the acyl carrier protein (ACP), a component of the fatty acid synthesis pathway. Thin layer chromatography (TLC) is a simple and easy way to prepare complex reaction samples for CMS analysis following a TLC/CMS approach.