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Application Note

Shotgun Lipidomics Analysis

Infusion based lipidomics analysis (shotgun Lipidomics) is an analysis strategy that involves the liquid extraction of lipids from samples such as blood, tissue homogenates or dried blood spots. This approach is suitable for a wide range of biomedical questions of lipid differentiation and lipid flux in the field of metabolic disease, neurological disorders, cancers or eye…

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Application Note

Microorganism Surface Analysis Using the TriVersa NanoMate for Liquid Extraction Surface Analysis of Bacteria and Fungi

Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS) has been utilized for determination of small molecules, lipids and proteins from a variety of surfaces such as tissue sections, plant material, medical devices, TLC plates or DBS cards. It allows direct surface analysis without sample preparation from spot sizes of 1 mm diameter by aspiration of extraction…

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Application Note

Pesticides from Food Surfaces Using LESA PLUS Liquid Extraction Surface Analysis PLUS LC Separation

Residues and contaminants in the food chain are an increasing problem due to high production volumes, large area distribution and import/export of food items across the world. The novel LESAPLUS surface analysis approach combines the standard liquid extraction surface analysis with an additional step of a nano liquid chromatography separation. This combination is ideally suited…

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Application Note

Lipid Species from Brain Tissue Sections Using LESA PLUS Liquid Extraction Surface Analysis PLUS LC Separation

Spatial lipid composition, distribution and regulation are very important factors for mediating lipid functionality and, when disrupted, can cause pathophysiological processes leading to cancer, obesity, atherosclerosis, and neurodegeneration. The novel LESAPLUS surface analysis approach combines the standard liquid extraction surface analysis with an additional step of a nano liquid chromatography separation. This combination is ideally…

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Application Note

Using the TriVersa NanoMate with LESA for Analyte Profiling in Material Science

Surface analysis techniques have gained significant attention over the last couple of years with LESA being an interesting alternative to techniques such as SIMS or MALDI imaging. This note demonstrates practical applications for LESA in the material sciences.

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Application Note

Metabolite Identification Using the TriVersa NanoMate®

Greater than 10 times more sensitive than LC/MS/MS for detection of low level, potentially active or toxic recirculating metabolites.

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Application Note

Lipid Profiling Using the TriVersa NanoMate®

Fast, reproducible, and automated lipid analysis using chip-based nanoelectrospray infusion.

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Application Note

Glycosylation Research Using the TriVersa NanoMate®

Fully characterize oligosaccharides that are missed by LC/MS/MS. Download this product note to find out how.

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Application Note

Using the TriVersa NanoMate® for Rapid Analyte Identification Based on Static nESI-MS/MS (Infusion MS/MS) Employing Polarity Switching Experiments

Automated static nano-ESI/MS/MS (Infusion MS/MS) is an easy-to-use and high-throughput approach to analyte identification. It is particularly useful in the field of shotgun lipidomics, which itself has great potential for biomarker discovery and gained some significant attention in the last couple of years (Schuhmann et al. 2012; Jung et al. 2011; Han et al. 2011).…

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Application Note

Using the TriVersa NanoMate® for Rapid Analyte Identification Based on Static nESI-MS/MS (Infusion MS/MS)

In many cases of routine protein identification, processing times become the major bottleneck at the core facility. Although a nano-LC/MS/MS approach is recognized as a general strategy for protein identification, the typical 30-120 min run-time prohibits fast turnaround times. The TriVersa NanoMate offers a more rapid approach for the infusion of the protein digest, with…

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Application Note

Non-covalent Interactions: Studying RNA – Small Molecule Binding Using Automated Chip-based Nanoelectrospray Mass Spectrometry in Negative Ion Mode

Negative ion nanoelectrospray ionization mass spectrometry (nESI-MS) is notoriously challenging, even more so under the high aqueous conditions required for protein-ligand interactions at physiological conditions. However, we present a successful application of negative ion nESI-MS for the investigation of noncovalent interactions between Ψ-RNA and small molecule inhibitors.

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