At regular intervals throughout the year a local lawn service applies pesticides to the lawn surrounding the Advion headquarters in Ithaca, NY. The grass is very uniform and not a single dandelion or crabgrass plant is visible – so we wondered, what chemical do they use? After an application in the early spring, we sampled…
In this application note, we demonstrate the use of the Advion expression CMS with a volatile APCI (vAPCI) ion source to analyze extra-virgin, virgin, and lampante olive oil samples. We show statistical analysis allowing us to categorize and identify olive oil samples by their grade. This technique allows us to test individual olive oil samples and…
Cheese is one of the world’s most popular food types, with a wide variety available for consumers. The scents and flavors of cheeses, so characteristic to each type of cheese, stem from a complex mixture of chemicals, including free fatty acids. While this mixture is affected by a wide variety of factors we can use…
In this application note, we demonstrate the use of the Advion expressionL CMS with an atmospheric solids analysis probe (ASAP) ion source to analyze two supplements (figure 1) that contain DMBA, a synthetic stimulant that has not been tested on humans. The substance is the chemical cousin to drug DBAA, which is currently banned by…
This application note demonstrates the use of the Advion expression CMS (compact mass spectrometer) coupled with a vapor APCI (vAPCI) ion source to directly detect several key chemicals given off of meat as it spoils at ambient temperature. Evolution of putrescine, cadaverine, and indole were measured over several days as meat spoiled.
The Advion deconvolution algorithm employs a combination of predictive charge envelope generation, overlap calculation with actual MS data acquired utilizing a maximum entropy goodness of fit approach followed by artifact removal through determination of peak significance. This novel approach allows for a rapid deconvolution over a wide mass range in seconds. It also allows a…
This application note suggests that a simple LC/MS screen of a relatively fresh sample of human whole blood collected from a lavender capped potassium EDTA anticoagulant blood sample applied to a cotton swab can be analyzed by full-scan positive ion or negative ion LC/MS using a compact single quadrupole mass spectrometer (expression CMS).
Reaction monitoring is a key aspect in a range of chemistry environments from chemical synthesis to drug discovery to understanding natural products and protein synthesis. To understand the optimal time to quench a reaction for maximum yield as well as to monitor a reaction real-time is vital to many medicinal and synthetic organic chemists. The…
Changtong Hao, Ph.D. – Advion, Inc. This application note will demonstrate the LC/MS determination of 16 PAHs (EPA 610 Mix) in the EPA priority target list using the Advion expression Compact Mass Spectrometer (CMS) coupled to an Agilent 1220 Liquid Chromatography (LC) system. The detection limits for the 16 PAHs in a water matrix were…
D. Tranchemontagne, N. Sousou, S. J. Prosser, J.D. Henion – Advion, Inc. & S. Orlowicz, S. Sadjadi, L. Snow – PhenoLogix The Advion expression CMS (compact mass spectrometer) coupled with Liquid Chromatography (LC) was used as a qualitative screening tool. This application note will demonstrate the screening of various drugs, including amphetamines, benzodiazepines, antidepressants, opiates…
Using Thin-Layer Chromatography with Compact Mass Spectrometry A sprig of mistletoe symbolizes a tradition of romance; however, reputed to be the “kiss of death,” mistletoe is said by some to be so poisonous that humans can be killed if they ingest the leaves or berries. In the spirit of the Holiday season and to ensure…
An office colleague has stolen a prized chocolate bar, and Dr. Eikel with the help of his partner, Dr. Henion, use an ASAP enabled expression CMS to determine whodunnit. This application note describes the process, and points the finger at the guilty culprit!
This note covers work carried out at Leeds University with co-workers from Durham University in the area of flow chemical synthesis using the expression CMS (Advion, Ithaca, NY). Two different reactions are described.
Co-authored with Merck Millipore in Darmstadt, Germany, this application note provides an example of TLC/CMS (thin-layer chromatography-compact mass spectrometry) for the determination of 4-aminobiphenyl in its reaction mixture.
Co-authored with Merck Millipore in Darmstadt, Germany, this application note demonstrates a TLC/CMS (thin-layer chromatography-compact mass spectrometry) technique for the rapid determination of 4-aminobiphenyl in its reaction mixture.
With the emergence of new protein therapeutics, including biosimilars, biobetters and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation. The purpose of this study is to provide guidelines for developing optimal LC running conditions with Aeris™…
Real-time reaction monitoring based on a combination of thin-layer chromatography (TLC) and compact mass spectrometry (CMS) is a simple and quick way for chemists to overcome synthetic challenges and optimize chemical reactions in the modern laboratory. Peptides of pharmaceutical interest can be readily synthesized following a rapid, continuous solution-phase synthesis strategy based on Fmoc protected…
The scientific investigation of cultural heritage objects is the only access to knowledge about long-gone, ancient societies and their cultural, technological as well as their social stage of development. In addition, the materials used by our ancestors, e.g. their textiles and the organic dyestuff to colorize these materials, allow researchers to draw some conclusions about…
Sudan Dyes are synthesized azo-dyes which are widely used to generate color in plastics, leather, fabrics, oil, waxes, etc. They are classified as Group 3 carcinogens by the International Agency for Research on Cancer (IARC) and are banned as food additives worldwide. However, in some countries these dyes are still occasionally used in order to…
This application note presents an online thin-layer chromatography (TLC)/CMS technique using the Advion expression compact mass spectrometer (CMS) and Plate ExpressTM interface to provide compound structural information without sample preparation after TLC separation. A Suzuki reaction for the synthesis of 4-aminobiphenyl will be demonstrated. The online TLC/CMS technique provides rapid and accurate determination of reaction…
Natural products, the underlying active components and their use in traditional medicine are of increased interest in the field of pharmaceutical research. The naturally occurring active ingredients are ideal chemical starting structures to improve upon in the pharmaceutical drug development process and consequently half of all currently approved drugs in the US are based on…
Analysis of the active components of the cannabis sativa plant is required both for law enforcement where these compounds are illegal and also as a tool for product control and optimization in the increasing number of legal markets for cannabis plant products. Whenever simple, unequivocal and legally defensible analysis methods for the detection and quantification…
The ability to effectively screen passengers with minimal personal contact is critical. Ion mobility detectors used at many airports can be inaccurate and provide many false-positives and false-negatives. Advanced Imaging Technology (AIT) screening techniques are very useful for detecting concealed weapons and explosive devices located on passengers and/or in their belongings, however are unable to…
This is an expanded application note with more data regarding direct sample analysis of THC in suspected contraband and on skin. Direct sample analysis is possible via an Atmospheric Solids Analysis Probe (ASAP®) combined with atmospheric pressure chemical ionization (APCI) on a compact mass spectrometer (CMS). This technique allows for the rapid investigation (<30 sec)…
Direct sample analysis is possible via an Atmospheric Solids Analysis Probe (ASAP®) combined with atmospheric pressure chemical ionization (APCI) on a compact mass spectrometer (CMS). This technique allows for the rapid investigation (<30 sec) of material suspected of containing THC/cannabinoids.
The expression CMS with the direct analysis probe offers chemists the ability to rapidly analyze solids, liquids and powders without tedious and time consuming sample preparation.
A combination of ion-ion interaction chromatography and a compact mass spectrometer presents a cost effective and powerful analysis system for the detection and quantification of amino acids in complex mixtures without the need for extra sample processing steps such as derivatizations.
Quantitation of Perchlorate by Ion Chromatography with Suppressed Conductivity on the expression CMS Perchlorate is a synthetic chemical that is used in the manufacture of rocket fuel, fireworks, flares and explosives, and can also be present in bleaches and some fertilizers [1]. The EPA has devised drinking water regulations for Perchlorate to minimize its impact…
The expression CMS provides essential compound information quickly and improves the chemist’s workflow. The Atmospheric Solids Analysis Probe (ASAP®) permits fast analysis of solid and liquid samples and is a simple, low cost alternative to LC/MS methods. This direct analysis probe method has been shown to be useful for the analysis of volatile and semivolatile…
The expression CMS provides essential compound information quickly and improves the chemist’s workflow. The Atmospheric Solids Analysis Probe (ASAP®) permits fast analysis of solid and liquid samples and is a simple, faster and lower cost alternative to LC/MS methods. This direct analysis probe method has been shown to be useful for the analysis of volatile…
Via Manual Injection using a Compact Mass Spectrometer with extended mass range (CMS-L) The CMS-L is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — ideal for large molecule applications such as the analysis of proteins. Its small foot print makes it the…
Using the expression CMS-L compact mass spectrometer The CMS-L is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — well-suited for large molecule applications such as the analysis of proteins. Its small foot print makes it the instrument of choice for space restricted…
Compact Mass Spectrometry for Fast, Quantitative VOC Screening Breath analysis is a valuable and widely-used technique that is gaining popularity in medical diagnostics, sports science, substance abuse and other fields where immediate, reliable detection of a variety of in-vivo compounds and their metabolites is required. However, the actual analysis can be complicated because of the type of…
The expression compact mass spectrometer (CMS) is a high performance, easy-to-use single quadrupole mass spectrometer with a small footprint to fit in space restricted labs. Priced substantially lower than other available systems, the CMS brings the analytical benefits of FIA/CMS and LC/CMS within the reach of more scientists than ever before.
The expression compact mass spectrometer (CMS) is a high performance, easy to use single quadrupole mass spectrometer with a small footprint to fit in space restricted labs. Priced substantially lower than other systems, the CMS brings the analytical benefits of mass spectrometry to more scientists than ever before.
The rapid identification of radiolabeled compounds would clearly be beneficial for applied clinical and preclinical tracers used for imaging with positron emission tomography (PET). The limited uptake of mass spectrometry in the imaging field has been due to several factors including concerns regarding adequate sensitivity, instrument footprint in the highly constrained space requirements for the…
Ultra (high) performance liquid chromatography (UPLC/UHPLC) is a relatively new technique, and is valued for its speed, efficiency and the quality of information provided. Many synthetic and medicinal chemistry laboratories are moving to this process to improve their workflows and save time.
Real-time reaction monitoring based on the expression compact mass spectrometer (CMS) can support the synthetic chemist to overcome synthesis challenges and optimize the reaction time on-line. Peptides of pharmaceutical interest can be readily synthesized following a rapid, continuous solution-phase synthesis strategy based on Fmoc protected amino acid building blocks 1. A simple model for such…
The Advion expression Compact Mass Spectrometer (CMS) was coupled to a Normal Phase Flash Chromatography system for mass directed fraction collection. Typically a UV detector is used to monitor compounds eluting from Normal Phase columns. A limitation of the UV detector is that it relies on compounds having a chromophore in the chemical structure, hence…
Recently, Advion co-authored with a major pharmaceutical partner to create two application notes highlighting the benefits of supercritical fluid chromatography (SFC) coupled to a compact mass spectrometer for mass directed fraction collection. The application notes conclude that mass‐directed fraction collection with a simplified passive splitter on the Preparative JASCO SFC coupled with expression CMS system…
Advion demonstrates a novel compact single quadrupole mass spectrometer for hood-based applications in the analysis of chemical reactions employing either LC/MS or flow injection (FIA).