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Application Note

Direct Analysis of Intact Proteins using the Advion Touch Express© Open Port Sampling Interface (OPSI) with the expressionL Compact Mass Spectrometer

Touch ExpressTM is a new sampling device for mass spec analysis using a technique developed by Gary Van Berkel and Vilmos Kertesz of Oak Ridge National Laboratory – the Open Port Sample Interface (OPSI). This fast, versatile sample inlet paired with the expression Compact Mass Spectrometer (CMS) provides simple molecular weight determination of surfaces, solids and liquids in < 30s.

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Application Note

Fast Identification of Artificial Vanilla Flavor in Vanilla Extract by ASAP® Compact Mass Spectrometry

The expression CMS coupled with the Atmospheric Solids Analysis Probe (ASAP®) is used to quickly identify the presence of artificial vanilla, as well as confirm the purity of products marketed as such. This technique provides a fast and easy method for purity analysis.

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Application Note

Fast Screening for Purity and Adulterants in Coconut Oil by ASAP® Compact Mass Spectrometry

The expression CMS coupled with the Atmospheric Solids Analysis Probe (ASAP®) is used to determine purity and detect adulterants in coconut oil. In this application note, pure coconut oil is tested against a sample of coconut oil adulterated with canola oil for comparative data analysis and a fast and easy method for purity analysis.

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Application Note

iASAP Analysis of Air-Sensitive Compounds via Inert Sampling Techniques

Many chemical reactions carried out by synthetic chemists involve air-sensitive compounds such as metal catalysts and organometallics and must therefore be carried out in a glove box or using a Schlenk line to prevent oxidation and hydrolysis. Sampling and transportation of these samples to a mass spectrometer by an Atmospheric Solids Analysis Probe (ASAP ®)…

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Application Note

Identification of a Pesticide Used in Lawn Care by vAPCI Compact Mass Spectrometry

At regular intervals throughout the year a local lawn service applies pesticides to the lawn surrounding the Advion headquarters in Ithaca, NY. The grass is very uniform and not a single dandelion or crabgrass plant is visible – so we wondered, what chemical do they use? After an application in the early spring, we sampled…

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Application Note

Analyses of Different Grades of Olive Oil by Volatile Mass Spectrometry

In this application note, we demonstrate the use of the Advion expression CMS with a volatile APCI (vAPCI) ion source to analyze extra-virgin, virgin, and lampante olive oil samples. We show statistical analysis allowing us to categorize and identify olive oil samples by their grade. This technique allows us to test individual olive oil samples and…

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Application Note

Classifying Cheeses by vAPCI Compact Mass Spectrometry

Cheese is one of the world’s most popular food types, with a wide variety available for consumers. The scents and flavors of cheeses, so characteristic to each type of cheese, stem from a complex mixture of chemicals, including free fatty acids. While this mixture is affected by a wide variety of factors we can use…

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Application Note

Screening of Dietary Supplements by ASAP® Compact Mass Spectrometry

In this application note, we demonstrate the use of the Advion expressionL CMS with an atmospheric solids analysis probe (ASAP) ion source to analyze two supplements (figure 1) that contain DMBA, a synthetic stimulant that has not been tested on humans. The substance is the chemical cousin to drug DBAA, which is currently banned by…

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Application Note

Chemically Tracking Spoilage of Meat Using Volatile Mass Spectrometry

This application note demonstrates the use of the Advion expression CMS (compact mass spectrometer) coupled with a volatile APCI (vAPCI) ion source to directly detect several key chemicals given off of meat as it spoils at ambient temperature. Evolution of putrescine, cadaverine, and indole were measured  over several days as meat spoiled.

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Application Note

Protein analysis via LC/CMS with automated charge state deconvolution

The Advion deconvolution algorithm employs a combination of predictive charge envelope generation, overlap calculation with actual MS data acquired utilizing a maximum entropy goodness of fit approach followed by artifact removal through determination of peak significance. This novel approach allows for a rapid deconvolution over a wide mass range in seconds. It also allows a…

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Application Note

LC/MS Screening For EDTA In Fresh Blood Swabs Using the Advion expression CMS System

This application note suggests that a simple LC/MS screen of a relatively fresh sample of human whole blood collected from a lavender capped potassium EDTA anticoagulant blood sample applied to a cotton swab can be analyzed by full-scan positive ion or negative ion LC/MS using a compact single quadrupole mass spectrometer (expression CMS).

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Application Note

Complete Reaction-Monitoring Solution

Reaction monitoring is a key aspect in a range of chemistry environments from chemical synthesis to drug discovery to understanding natural products and protein synthesis. To understand the optimal time to quench a reaction for maximum yield as well as to monitor a reaction real-time is vital to many medicinal and synthetic organic chemists. The…

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Application Note

LC/CMS Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in Water (EPA 610 mix)

Changtong Hao, Ph.D. – Advion, Inc. This application note will demonstrate the LC/MS determination of 16 PAHs (EPA 610 Mix) in the EPA priority target list using the Advion expression Compact Mass Spectrometer (CMS) coupled to an Agilent 1220 Liquid Chromatography (LC) system. The detection limits for the 16 PAHs in a water matrix were…

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Application Note

Qualitative Analysis of Commonly Abused Drugs by LC/MS on the expression CMS

D. Tranchemontagne, N. Sousou, S. J. Prosser, J.D. Henion – Advion, Inc. & S. Orlowicz, S. Sadjadi, L. Snow – PhenoLogix The Advion expression CMS (compact mass spectrometer) coupled with Liquid Chromatography (LC) was used as a qualitative screening tool. This application note will demonstrate the screening of various drugs, including amphetamines, benzodiazepines, antidepressants, opiates…

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Application Note

Mistletoe: Kiss of Love or Death?

Using Thin-Layer Chromatography with Compact Mass Spectrometry A sprig of mistletoe symbolizes a tradition of romance; however, reputed to be the “kiss of  death,” mistletoe is said by some to be so poisonous that humans can be killed if they  ingest the leaves or berries. In the spirit of the Holiday season and to ensure…

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Application Note

To Catch the Candy Thief: Who stole my chocolate?

An office colleague has stolen a prized chocolate bar, and Dr. Eikel with the help of his partner, Dr. Henion, use an ASAP enabled expression CMS to determine whodunnit. This application note describes the process, and points the finger at the guilty culprit!

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Application Note

Flow Chemistry Monitoring & Optimization Using Compact Mass Spectrometry

This note covers work carried out at Leeds University with co-workers from Durham University in the area of flow chemical synthesis using the expression CMS (Advion, Ithaca, NY). Two different reactions are described.

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Application Note

Analysis of 4-Aminobiphenyl and 4-Bromoanilin on normal phase TLC using TLC/MS

Co-authored with Merck Millipore in Darmstadt, Germany, this application note provides an example of TLC/CMS (thin-layer chromatography-compact mass spectrometry) for the determination of 4-aminobiphenyl in its reaction mixture.

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Application Note

Analysis of 4-Aminobiphenyl and 4-Bromoanilin on reversed phase TLC using TLC/MS

Co-authored with Merck Millipore in Darmstadt, Germany, this application note demonstrates a TLC/CMS (thin-layer chromatography-compact mass spectrometry) technique for the rapid determination of 4-aminobiphenyl in its reaction mixture.

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Application Note

Recommendations for Transferring Peptide Mapping Methods to LC/MS Using Core-Shell Columns

With the emergence of new protein therapeutics, including biosimilars, biobetters and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation. The purpose of this study is to provide guidelines for developing optimal LC running conditions with Aeris™…

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Application Note

Real-Time Reaction Monitoring of a Solution-Phase Peptide Synthesis using the PlateExpress and expression TLC/CMS

Real-time reaction monitoring based on a combination of thin-layer chromatography (TLC) and compact mass spectrometry (CMS) is a simple and quick way for chemists to overcome synthetic challenges and optimize chemical reactions in the modern laboratory.  Peptides of pharmaceutical  interest can be readily synthesized following a rapid, continuous solution-phase synthesis strategy based on Fmoc protected…

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Application Note

Dyestuff Analysis of Cultural Heritage Objects by Direct Analysis Probe on a Compact Mass Spectrometer

The scientific investigation of cultural heritage objects is the only access to knowledge about long-gone, ancient societies and their cultural, technological as well as their social  stage of development. In addition, the materials used by our ancestors, e.g. their textiles and the organic dyestuff to colorize these materials, allow researchers to draw some conclusions about…

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Application Note

Sudan Dyes Analysis Using Compact Mass Spectrometry with TLC Interface

Sudan Dyes are synthesized azo-dyes which are widely used to generate color in plastics, leather, fabrics, oil, waxes, etc. They are classified as Group 3 carcinogens by the International Agency for Research on Cancer (IARC) and are banned as food additives worldwide. However, in some countries these dyes are still occasionally used in order to…

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Application Note

Suzuki Reaction Monitoring Using Compact Mass Spectrometry with TLC Interface

This application note presents an online thin-layer chromatography (TLC)/CMS technique using the Advion expression compact mass spectrometer (CMS) and Plate ExpressTM interface to provide compound structural information without sample preparation after TLC separation. A Suzuki reaction for the synthesis of 4-aminobiphenyl will be demonstrated. The online TLC/CMS technique provides rapid and accurate determination of reaction…

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Application Note

Natural Product Analysis Using Compact Mass Spectrometry with Polarity Switching and In-source CID

Natural products, the underlying active components and their use in traditional medicine are of increased interest in the field of pharmaceutical research. The naturally occurring active ingredients are ideal chemical starting structures to improve upon in the  pharmaceutical drug development process and consequently half of all currently approved  drugs in the US are based on…

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Application Note

THC Plant Material Analysis Using Compact Mass Spectrometry for Product Control and Law Enforcement of cannabis-related Plant Products

Analysis of the active components of the cannabis sativa plant is required both for law enforcement where these compounds are illegal and also as a tool for product control and optimization in the increasing number of legal markets for cannabis plant products. Whenever simple, unequivocal and legally defensible  analysis methods for the detection and quantification…

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Application Note

Screening of explosives residues without sample preparation using a direct sample analysis probe on the expression CMS

The ability to effectively screen passengers with minimal personal contact is critical. Ion mobility detectors used at many airports can be inaccurate and provide many false-positives and false-negatives. Advanced Imaging Technology (AIT) screening techniques are very useful for detecting concealed weapons and explosive devices located on passengers and/or in their belongings, however are unable to…

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Application Note

Direct Sample Analysis: Detecting THC/Cannabinoids in Contraband by Compact Mass Spectrometry

This is an expanded application note with more data regarding direct sample analysis of THC in suspected contraband and on skin. Direct sample analysis is possible via an Atmospheric Solids Analysis Probe (ASAP®) combined with atmospheric pressure chemical ionization (APCI) on a compact mass spectrometer (CMS). This technique allows for the rapid investigation (<30 sec)…

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Application Note

Direct Sample Analysis: Detecting THC/Cannabinoids in Contraband by Compact Mass Spectrometry

Direct sample analysis is possible via an Atmospheric Solids Analysis Probe (ASAP®) combined with atmospheric pressure chemical ionization (APCI) on a compact mass spectrometer (CMS). This technique allows for the rapid investigation (<30 sec) of material suspected of containing THC/cannabinoids.

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Application Note

Direct Sample Analysis of “Fizzy Drinks”

The expression CMS with the direct analysis probe offers chemists the ability to rapidly analyze solids, liquids and powders without tedious and time consuming sample preparation.

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Application Note

LC/MS Determination of Amino Acids Using Compact Mass Spectrometry

A combination of ion-ion interaction chromatography and a compact mass spectrometer presents a cost effective and powerful analysis system for the detection and quantification of amino acids in complex mixtures without the need for extra sample processing steps such as derivatizations.

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Application Note

Environmental Analysis

Quantitation of Perchlorate by Ion Chromatography with Suppressed Conductivity on the expression CMS Perchlorate is a synthetic chemical that is used in the manufacture of rocket fuel, fireworks, flares and explosives, and can also be present in bleaches and some fertilizers [1]. The EPA has devised drinking water regulations for Perchlorate to minimize its impact…

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Application Note

Analysis of Two Reaction Products by Direct Analysis Probe on a Compact Mass Spectrometer

The expression CMS provides essential compound information quickly and improves the chemist’s workflow. The Atmospheric Solids Analysis Probe (ASAP®) permits fast analysis of solid and liquid samples and is a simple, low cost alternative to LC/MS methods. This direct analysis probe method has been shown to be useful for the analysis of volatile and semivolatile…

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Application Note

Reaction Monitoring by Direct Analysis Probe on a Compact Mass Spectrometer

The expression CMS provides essential compound information quickly and improves the chemist’s workflow. The Atmospheric Solids Analysis Probe (ASAP®) permits fast analysis of solid and liquid samples and is a simple, faster and lower cost alternative to LC/MS methods. This direct analysis probe method has been shown to be useful for the analysis of volatile…

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Application Note

Protein Analysis

Via Manual Injection using a Compact Mass Spectrometer with extended mass range (CMS-L) The CMS-L is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — ideal for large molecule applications such as the analysis of proteins. Its small foot print makes it the…

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Application Note

LC/MS Analysis of Proteins

Using the expression CMS-L compact mass spectrometer The CMS-L is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — well-suited for large molecule applications such as the analysis of proteins. Its small foot print makes it the instrument of choice for space restricted…

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Application Note

Ambient Ionization

Compact Mass Spectrometry for Fast, Quantitative VOC Screening Breath analysis is a valuable and widely-used technique that is gaining popularity in medical diagnostics, sports science, substance abuse and other fields where immediate, reliable detection of a variety of in-vivo compounds and their metabolites is required. However, the actual analysis can be complicated because of the type of…

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Application Note

Peptide/Protein Analysis in Less than One Minute

The expression compact mass spectrometer (CMS) is a high performance, easy-to-use single quadrupole mass spectrometer with a small footprint to fit in space restricted labs. Priced substantially lower than other available systems, the CMS brings the analytical benefits of FIA/CMS and LC/CMS within the reach of more scientists than ever before.

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Application Note

Protein Analysis Using Compact Mass Spectrometry

The expression compact mass spectrometer (CMS) is a high performance, easy to use single quadrupole mass spectrometer with a small footprint to fit in space restricted labs. Priced substantially lower than other systems, the CMS brings the analytical benefits of mass spectrometry to more scientists than ever before.

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Application Note

Routine detection and identification of PET radiopharmaceuticals

The rapid identification of radiolabeled compounds would clearly be beneficial for applied clinical and preclinical tracers used for imaging with positron emission tomography (PET). The limited uptake of mass spectrometry in the imaging field has been due to several factors including concerns regarding adequate sensitivity, instrument footprint in the highly constrained space requirements for the…

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Application Note

Improved efficiency and faster answers — Compact mass spectrometry and U(H)PLC

Ultra (high) performance liquid chromatography (UPLC/UHPLC) is a relatively new technique, and is valued for its speed, efficiency and the quality of information provided. Many synthetic and medicinal chemistry laboratories are moving to this process to improve their workflows and save time.

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Application Note

Real Time Reaction Monitoring of a Solution Phase Peptide Synthesis

Real-time reaction monitoring based on the expression compact mass spectrometer (CMS) can support the synthetic chemist to overcome synthesis challenges and optimize the reaction time on-line. Peptides of pharmaceutical interest can be readily synthesized following a rapid, continuous solution-phase synthesis strategy based on Fmoc protected amino acid building blocks 1. A simple model for such…

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Application Note

Mass Directed Fraction Collection

The Advion expression Compact Mass Spectrometer (CMS) was coupled to a Normal Phase Flash Chromatography system for mass directed fraction collection. Typically a UV detector is used to monitor compounds eluting from Normal Phase columns. A limitation of the UV detector is that it relies on compounds having a chromophore in the chemical structure, hence…

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Application Note

Simple, high-performance coupling of SFC to CMS for mass-directed fraction collection

Recently, Advion co-authored with a major pharmaceutical partner to create two application notes highlighting the benefits of supercritical fluid chromatography (SFC) coupled to a compact mass spectrometer for mass directed fraction collection.  The application notes conclude that mass‐directed fraction collection with a simplified passive splitter on the Preparative JASCO SFC coupled with expression CMS system…

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Application Note

Real-time monitoring of Suzuki reaction using compact mass spectrometry via LC/MS and flow injection (FI)/MS

Advion demonstrates a novel compact single quadrupole mass spectrometer for hood-based applications in the analysis of chemical reactions employing either LC/MS or flow injection (FIA).

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