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Application Note

Protein analysis via LC/CMS with automated charge state deconvolution

The Advion deconvolution algorithm employs a combination of predictive charge envelope generation, overlap calculation with actual MS data acquired utilizing a maximum entropy goodness of fit approach followed by artifact removal through determination of peak significance. This novel approach allows for a rapid deconvolution over a wide mass range in seconds. It also allows a…

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Application Note

Complete Reaction-Monitoring Solution

Reaction monitoring is a key aspect in a range of chemistry environments from chemical synthesis to drug discovery to understanding natural products and protein synthesis. To understand the optimal time to quench a reaction for maximum yield as well as to monitor a reaction real-time is vital to many medicinal and synthetic organic chemists. The…

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Application Note

Recommendations for Transferring Peptide Mapping Methods to LC/MS Using Core-Shell Columns

With the emergence of new protein therapeutics, including biosimilars, biobetters and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation. The purpose of this study is to provide guidelines for developing optimal LC running conditions with Aeris™…

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Application Note

Real-Time Reaction Monitoring of a Solution-Phase Peptide Synthesis using the PlateExpress and expression TLC/CMS

Real-time reaction monitoring based on a combination of thin-layer chromatography (TLC) and compact mass spectrometry (CMS) is a simple and quick way for chemists to overcome synthetic challenges and optimize chemical reactions in the modern laboratory.  Peptides of pharmaceutical  interest can be readily synthesized following a rapid, continuous solution-phase synthesis strategy based on Fmoc protected…

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Application Note

LC/MS Determination of Amino Acids Using Compact Mass Spectrometry

A combination of ion-ion interaction chromatography and a compact mass spectrometer presents a cost effective and powerful analysis system for the detection and quantification of amino acids in complex mixtures without the need for extra sample processing steps such as derivatizations.

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Application Note

Protein Analysis

Via Manual Injection using a Compact Mass Spectrometer with extended mass range (CMS-L) The CMS-L is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — ideal for large molecule applications such as the analysis of proteins. Its small foot print makes it the…

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Application Note

LC/MS Analysis of Proteins

Using the expression CMS-L compact mass spectrometer The CMS-L is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — well-suited for large molecule applications such as the analysis of proteins. Its small foot print makes it the instrument of choice for space restricted…

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Application Note

Peptide/Protein Analysis in Less than One Minute

The expression compact mass spectrometer (CMS) is a high performance, easy-to-use single quadrupole mass spectrometer with a small footprint to fit in space restricted labs. Priced substantially lower than other available systems, the CMS brings the analytical benefits of FIA/CMS and LC/CMS within the reach of more scientists than ever before.

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Application Note

Protein Analysis Using Compact Mass Spectrometry

The expression compact mass spectrometer (CMS) is a high performance, easy to use single quadrupole mass spectrometer with a small footprint to fit in space restricted labs. Priced substantially lower than other systems, the CMS brings the analytical benefits of mass spectrometry to more scientists than ever before.

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Application Note

Real Time Reaction Monitoring of a Solution Phase Peptide Synthesis

Real-time reaction monitoring based on the expression compact mass spectrometer (CMS) can support the synthetic chemist to overcome synthesis challenges and optimize the reaction time on-line. Peptides of pharmaceutical interest can be readily synthesized following a rapid, continuous solution-phase synthesis strategy based on Fmoc protected amino acid building blocks 1. A simple model for such…

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