Protein Identification in Imaging Mass Spectrometry through Spatially Targeted Liquid Micro-Extractions featuring the Advion TriVersa NanoMate® LESA® Plus

Daniel J. Ryan, David Nei, Boone M. Prentice, Kristie L. Rose, Richard M. Caprioli, Jeffrey M. Spraggins

Robotic liquid surface extractions can be used to interrogate discrete regions of tissue to provide protein identifications with high throughput, accuracy, and robustness. The direct coupling of tissue surface extractions and liquid chromatography, offers a new and effective approach to provide spatial proteomics data in an imaging experiment.

Tissue extractions were completed using the TriVersa NanoMate® (Advion, Inc., Ithaca, NY, USA) modified to include a glass capillary (LESA Plus) for improved spatial resolution and online integration with LC-based experiments. 36 Scanned images of thaw-mounted samples were uploaded to the ChipSoft Software (Advion, Inc.) to allow histological regions of interest to be selected for analysis.