Mode 3: Liquid Extraction Surface Analysis (LESA®)

Advion TriVersa LESA

Liquid Extraction Surface Analysis (LESA®) was developed in collaboration  with Dr. Gary van Berkel from the Oak Ridge National Laboratories. Its unique surface extraction via a liquid droplet held in suspension (liquid junction) allows a novel approach for spatially resolved analysis of biological and technical  surfaces of interest. The pipetting accuracy of the TriVersa NanoMate enables high sensitivity experiments with a spatial resolution of 1mm on target.

[ Animation: The TriVersa NanoMate for LESA ]

[ Video: LESA in action presented by Dr. Jack Henion ]

The LESA capability is also available as an upgrade to all previously installed TriVersa NanoMate systems.

Typical Applications

  • PK/PD analysis of small molecule drugs
  • Spatially resolved lipidomics
  • Biofilm analysis from medical devices
  • Analysis of bacteria and fungi from culture
  • Direct protein analysis
  • Plant material


  • Fast, simple and direct analysis
  • 1 mm spatial resolution
  • Liquid extraction and nESI based analysis
  • High sensitivity due to nESI and extraction into only 1 µL of solvent

TriVersa NanoMate Success Stories

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University of Southern Denmark

"Essential for any Lipidomics Laboratory."

Christer S. Ejsing, PhD, Associate Professor

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University of Birmingham

"The TriVersa NanoMate revolutionized nanospray."

Dr. Helen Cooper, Senior Lecturer, School of BioSciences

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Max Planck Institute of Molecular Plant Physiology Golm, Germany

"I can’t imagine running these analyses without the TriVersa NanoMate"

Dr. Patrick Giavalisco, Research Group Leader

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School of Chemistry, University of Bristol – Bristol, UK

"Advion’s technology gives you what you want very quickly."

John Crosby, Senior Lecturer, Organic & Biological Chemistry

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Recent TriVersa NanoMate Publications

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TriVersa NanoMate Application Notes

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Shotgun Lipidomics Analysis

Infusion based lipidomics analysis (shotgun Lipidomics) is an analysis strategy that involves the liquid extraction of lipids from samples such as blood, tissue homogenates or dried blood spots. This approach is suitable for a wide range of biomedical questions of lipid differentiation and lipid flux in the field of metabolic disease, neurological disorders, cancers or eye…

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Microorganism Surface Analysis Using the TriVersa NanoMate for Liquid Extraction Surface Analysis of Bacteria and Fungi

Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS) has been utilized for determination of small molecules, lipids and proteins from a variety of surfaces such as tissue sections, plant material, medical devices, TLC plates or DBS cards. It allows direct surface analysis without sample preparation from spot sizes of 1 mm diameter by aspiration of extraction…

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Pesticides from Food Surfaces Using LESA PLUS Liquid Extraction Surface Analysis PLUS LC Separation

Residues and contaminants in the food chain are an increasing problem due to high production volumes, large area distribution and import/export of food items across the world. The novel LESAPLUS surface analysis approach combines the standard liquid extraction surface analysis with an additional step of a nano liquid chromatography separation. This combination is ideally suited…

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Using the TriVersa NanoMate with LESA for Analyte Profiling in Material Science

Surface analysis techniques have gained significant attention over the last couple of years with LESA being an interesting alternative to techniques such as SIMS or MALDI imaging. This note demonstrates practical applications for LESA in the material sciences.

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