Mode 2: LC/MS with Fraction Collection for Re-Analysis by Infusion

Mode 2 was developed to operate the system as a nano-ESI ion source for LC/MS applications. In addition, excess LC eluate can be utilized for fraction collection to create a physical storage of the LC/MS experiment and allow re-analysis of fractions of interest as identified by the online LC/MS run.

Mode 2 is ideally suited for researchers interested in metabolite identification, biomarker analysis, and protein characterization or any application that requires more time for analysis than what is allowed during conventional liquid chromatography MS analysis.

[ Animation: How Simultaneous LC/MS fraction collection works ]

Users can couple any conventional HPLC system to the TriVersa NanoMate® LESA® for MS analysis (optional RAD or UV detectors can also be utilized). The effluent is split post column with a small portion directed to the Advion ESI Chip® for ionization and LC/MS analysis while the remainder of the split effluent is collected into a multi-well plate. The chromatogram is thereby “stored” for future analysis with each fraction assigned a timestamp of the LC/MS analysis. Following LC/MS, the researcher is able to select fractions of interest for more in-depth analysis via Infusion MSn.

Typical Applications

  • Metabolite Identification
  • Protein Biomarker Discovery
  • QA/QC of Antibodies
  • Top-down/bottom-up Proteomics
  • Protein PTM analysis

Benefits

  • More time across an LC/MS peak for more information from complex samples
  • Qualitative and quantitative information from a single LC/MS run
  • Integration with RAD or UV detectors and external fraction collectors for increased workflow flexibility

TriVersa NanoMate Success Stories

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University of Southern Denmark

"Essential for any Lipidomics Laboratory."

Christer S. Ejsing, PhD, Associate Professor

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University of Birmingham

"The TriVersa NanoMate revolutionized nanospray."

Dr. Helen Cooper, Senior Lecturer, School of BioSciences

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Max Planck Institute of Molecular Plant Physiology Golm, Germany

"I can’t imagine running these analyses without the TriVersa NanoMate"

Dr. Patrick Giavalisco, Research Group Leader

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School of Chemistry, University of Bristol – Bristol, UK

"Advion’s technology gives you what you want very quickly."

John Crosby, Senior Lecturer, Organic & Biological Chemistry

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Recent TriVersa NanoMate Publications

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TriVersa NanoMate Application Notes

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Shotgun Lipidomics Analysis

Infusion based lipidomics analysis (shotgun Lipidomics) is an analysis strategy that involves the liquid extraction of lipids from samples such as blood, tissue homogenates or dried blood spots. This approach is suitable for a wide range of biomedical questions of lipid differentiation and lipid flux in the field of metabolic disease, neurological disorders, cancers or eye…

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Microorganism Surface Analysis Using the TriVersa NanoMate for Liquid Extraction Surface Analysis of Bacteria and Fungi

Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS) has been utilized for determination of small molecules, lipids and proteins from a variety of surfaces such as tissue sections, plant material, medical devices, TLC plates or DBS cards. It allows direct surface analysis without sample preparation from spot sizes of 1 mm diameter by aspiration of extraction…

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Pesticides from Food Surfaces Using LESA PLUS Liquid Extraction Surface Analysis PLUS LC Separation

Residues and contaminants in the food chain are an increasing problem due to high production volumes, large area distribution and import/export of food items across the world. The novel LESAPLUS surface analysis approach combines the standard liquid extraction surface analysis with an additional step of a nano liquid chromatography separation. This combination is ideally suited…

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Using the TriVersa NanoMate with LESA for Analyte Profiling in Material Science

Surface analysis techniques have gained significant attention over the last couple of years with LESA being an interesting alternative to techniques such as SIMS or MALDI imaging. This note demonstrates practical applications for LESA in the material sciences.

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