Mode 1: Static Nano-Electrospray (Infusion)

The TriVersa NanoMate® is the only automated ion source platform that allows high throughput infusion experiments for small or large analytes in applications ranging from shotgun lipidomics, to non-covalent interaction studies (NCI), to protein characterization.

Operating in Mode 1: Infusion, the system utilizes the Advion ESI Chip® providing long, stable sprays, and eliminating sample-to-sample carryover.

[ Animation: How automated chip-based infusion works ]

Typical Applications

  • Lipidomics, Metabolomics
  • Non-covalent Interaction Studies (NCI)
  • Top-down Protein Identification and Characterization
  • QA/QC of Antibodies
  • Glycan Analysis


  • Zero sample-to-sample carryover due to one tip-one sample-one sprayer workflow
  • Highly efficient, automated nano-electrospray
  • Long, stable sprays from µL of sample
  • High sample throughput from 96, 384 or 1536 well plates

TriVersa NanoMate Success Stories

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University of Southern Denmark

"Essential for any Lipidomics Laboratory."

Christer S. Ejsing, PhD, Associate Professor

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University of Birmingham

"The TriVersa NanoMate revolutionized nanospray."

Dr. Helen Cooper, Senior Lecturer, School of BioSciences

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Max Planck Institute of Molecular Plant Physiology Golm, Germany

"I can’t imagine running these analyses without the TriVersa NanoMate"

Dr. Patrick Giavalisco, Research Group Leader

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School of Chemistry, University of Bristol – Bristol, UK

"Advion’s technology gives you what you want very quickly."

John Crosby, Senior Lecturer, Organic & Biological Chemistry

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Recent TriVersa NanoMate Publications

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TriVersa NanoMate Application Notes

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Shotgun Lipidomics Analysis

Infusion based lipidomics analysis (shotgun Lipidomics) is an analysis strategy that involves the liquid extraction of lipids from samples such as blood, tissue homogenates or dried blood spots. This approach is suitable for a wide range of biomedical questions of lipid differentiation and lipid flux in the field of metabolic disease, neurological disorders, cancers or eye…

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Microorganism Surface Analysis Using the TriVersa NanoMate for Liquid Extraction Surface Analysis of Bacteria and Fungi

Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS) has been utilized for determination of small molecules, lipids and proteins from a variety of surfaces such as tissue sections, plant material, medical devices, TLC plates or DBS cards. It allows direct surface analysis without sample preparation from spot sizes of 1 mm diameter by aspiration of extraction…

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Pesticides from Food Surfaces Using LESA PLUS Liquid Extraction Surface Analysis PLUS LC Separation

Residues and contaminants in the food chain are an increasing problem due to high production volumes, large area distribution and import/export of food items across the world. The novel LESAPLUS surface analysis approach combines the standard liquid extraction surface analysis with an additional step of a nano liquid chromatography separation. This combination is ideally suited…

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Using the TriVersa NanoMate with LESA for Analyte Profiling in Material Science

Surface analysis techniques have gained significant attention over the last couple of years with LESA being an interesting alternative to techniques such as SIMS or MALDI imaging. This note demonstrates practical applications for LESA in the material sciences.

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