Protein assays and peptide synthesis

Fast separation and mass analysis of proteins and peptides

The ’tides express combines an extended mass range, compact mass spectrometer (expression L CMS) with an easy-to-use HPLC gradient system with manual sample injector for rapid, real-time analysis of large molecules at the point-of-need. Additional fragmentation analysis and charge deconvolution software tools provide the interpretation needed to easily confirm the identity of peptides and proteins.

  • Cost-effective LC-CMS analysis tool ideally suited to support solid-phase peptide synthesis (SPPS) analytical needs
  • Provides both the analyte mass-to-charge ratio as well as structural and AA sequence information
  • Ensure quality control by including a reaction control step after each synthesis cycle to guide yield optimization efforts

expression CMS Success Stories

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Virginia Tech

"There is no substitute for having a mass spectrometer a few steps away from your bench."

John Matson, PhD, Assistant Professor

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Recent expression CMS Publications

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expression CMS Application Notes

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Protein analysis via LC/CMS with automated charge state deconvolution

The Advion deconvolution algorithm employs a combination of predictive charge envelope generation, overlap calculation with actual MS data acquired utilizing a maximum entropy goodness of fit approach followed by artifact removal through determination of peak significance. This novel approach allows for a rapid deconvolution over a wide mass range in seconds. It also allows a…

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Recommendations for Transferring Peptide Mapping Methods to LC/MS Using Core-Shell Columns

With the emergence of new protein therapeutics, including biosimilars, biobetters and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation. The purpose of this study is to provide guidelines for developing optimal LC running conditions with Aeris™…

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LC/MS Determination of Amino Acids Using Compact Mass Spectrometry

A combination of ion-ion interaction chromatography and a compact mass spectrometer presents a cost effective and powerful analysis system for the detection and quantification of amino acids in complex mixtures without the need for extra sample processing steps such as derivatizations.

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Protein Analysis

Via Direct Injection using a Compact Mass Spectrometer with extended mass range (CMSL) The CMSL is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — ideal for large molecule applications such as the analysis of proteins. Its small footprint makes it the instrument…

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