Mode 2: LC/MS fraction Collection/Infusion

LC/MS Fraction Collection using the TriVersa NanoMate gives researchers the ability to spend more time analyzing complex portions of their samples without having to reinject. LC/MS with simultaneous fraction collection is ideally suited for researchers interested in metabolite identification, biomarker analysis, and protein characterization or any application that requires more time for analysis than what is allowed during conventional liquid chromatography MS analysis.[ Animation: How Simultaneous LC/MS fraction collection works ]

In liquid chromatography/mass spectrometry (LC/MS) fraction collection mode, users couple any conventional HPLC system to the TriVersa NanoMate for MS analysis. The effluent is split post column with a portion directed to the ESI Chip® for ionization and LC/MS analysis while the remainder of the split effluent is collected into a multi-well plate. The chromatogram is thereby “stored” for future analysis with each fraction assigned a timestamp of the LC/MS analysis. Following LC/MS, the researcher is able to select fractions of interest for more in-depth analysis via Infusion MSn.

TriVersa NanoMate Success Stories

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University of Southern Denmark

"Essential for any Lipidomics Laboratory."

Christer S. Ejsing, PhD, Associate Professor

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University of Birmingham

"The TriVersa NanoMate revolutionized nanospray."

Dr. Helen Cooper, Senior Lecturer, School of BioSciences

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Max Planck Institute of Molecular Plant Physiology Golm, Germany

"I can’t imagine running these analyses without the TriVersa NanoMate"

Dr. Patrick Giavalisco, Research Group Leader

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School of Chemistry, University of Bristol – Bristol, UK

"Advion’s technology gives you what you want very quickly."

John Crosby, Senior Lecturer, Organic & Biological Chemistry

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Recent TriVersa NanoMate Publications

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TriVersa NanoMate Application Notes

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Microorganism Surface Analysis Using the TriVersa NanoMate for Liquid Extraction Surface Analysis of Bacteria and Fungi

Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS) has been utilized for determination of small molecules, lipids and proteins from a variety of surfaces such as tissue sections, plant material, medical devices, TLC plates or DBS cards. It allows direct surface analysis without sample preparation from spot sizes of 1 mm diameter by aspiration of extraction…

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Pesticides from Food Surfaces Using LESA PLUS Liquid Extraction Surface Analysis PLUS LC Separation

Residues and contaminants in the food chain are an increasing problem due to high production volumes, large area distribution and import/export of food items across the world. The novel LESAPLUS surface analysis approach combines the standard liquid extraction surface analysis with an additional step of a nano liquid chromatography separation. This combination is ideally suited…

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Using the TriVersa NanoMate with LESA for Analyte Profiling in Material Science

Surface analysis techniques have gained significant attention over the last couple of years with LESA being an interesting alternative to techniques such as SIMS or MALDI imaging. This note demonstrates practical applications for LESA in the material sciences.

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