In liquid chromatography/mass spectrometry
(LC/MS) fraction collection mode, the TriVersa NanoMate allows users to
couple any conventional HPLC system to the TriVersa NanoMate for MS analysis.
The effluent is split post column with a portion directed to a conventional
LC/MS interface while the remainder of the split effluent is directed
to collect at time segments into a multi-well plate. The chromatogram
is thereby “cataloged” for future analysis. Following
LC/MS, the researcher is able to select fractions of interest for more
in-depth analysis via MS/MS. The process gives researchers the ability
to spend more time analyzing complex portions of their samples without
having to reinject. LC/MS with simultaneous fraction collection
is ideally suited for researchers interested in metabolite
identification, biomarker discovery,
and protein characterization
or any application that requires more time for analysis than what is allowed
during conventional LC/MS.
Presented by Thomas Covey, Principal Research Scientist
of AB SCIEX
Fast. Simple.
Direct.
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analytes with better sensitivity by combining LESA with
mass spectrometry and chip-based nanoESI via Advion’s TriVersa NanoMate.
Applications include sample analysis from a variety of surfaces e.g.
