Validated Methods

LC/MS Biomarkers | Immunoassay Biomarkers | LC/MS Drug Asssays

Non-proprietary validated bioanalytical methods offer a cost-effective and timely solution for biomarker studies, drug-drug interaction (DDI) studies, or comparison studies with established therapeutic compounds. Advion is happy to partner with new and existing clients to develop non-proprietary assays that have value to the pharmaceutical industry.

Advion specializes in method development and validation of proprietary methods for our pharmaceutical partners worldwide. Advion also has a variety of validated LC/MS Biomarkers, Immunoassay Biomarkers, and LC/MS Drug Assays available that are non-proprietary (see below). Click on the validated method name to request additional information such as the anticoagulant used, accuracy and precision, and stability details for the assay.

If there is a non-proprietary method you feel we should offer, please email us at info@advion.com.

Click here for a PDF of our Validated Methods as described below.

LC/MS Biomarker Methods

Biomarker Method Development and Validation Expertise

Click here to request more information regarding our LC/MS Biomarker Methods.

Amino Acids

Lipids

Neurotransmitters

Nucleotides

Peptides

Immunoassay Biomarker Methods

Biomarker/Analyte on ELISA Platform	Range	Volume Required	Matrix
HER2/neu	2.5 - 35 ng/mL	20 µL	Human serum
IGF-1 (total)	0.15 - 6 ng/mL	20 µL	Human serum
IGF-1 (free)	0.15 - 6 ng/mL	20 µL	Human serum
sECAD	0.625 - 20 ng/mL	20 µL	Human serum
sEGFr	12.5 - 300 ng/mL	20 µL	Human serum

LC/MS/MS Methods

Analyte/Metabolite	Range	Extraction Volume	Matrix
Acyclovir/Valaciclovir	10 - 1000 ng/mL	50 µL	Human plasma
Amlodipine	0.1 - 10 ng/mL	100 µL	Human plasma
D-Amphetamine, L-Amphetamine (chiral)	0.5 - 75 ng/mL	200 µL	Human plasma
Atomoxetine	0.5 - 500 ng/mL	50 µL	Human plasma
Atorvastatin/o-Hydroxyatorvastatin; p-Hydroxyatorvastatin	0.25 - 25 ng/mL	200 µL	Human plasma
Bortezomib	0.1 - 25 ng/mL	25 µL	Human plasma
Capecitabine/5'-DFCR; 5'-DFUR; 5-FU; FBAL	10 - 5000 ng/mL 50 - 25000 ng/mL 2 - 1000 ng/mL 15 - 7500 ng/mL	200 µL	Human plasma
Carbamazepine/ Carbamazepine-10, 11 epoxide	20 - 10000 ng/mL 10 - 2000 ng/mL	TBD	Human plasma
Available in June 2010 Clopidogrel; Clopidogrel Carboxylic Acid; Clopidogrel Active Metabolite	0.5 - 50 ng/mL 5 - 5000 ng/mL 0.1 - 100 ng/mL	200 µL	Human plasma
Available in June 2010 Cytarabine	25 - 1000 ng/mL	TBD	Human plasma
Daunorubicin/Daunorubicinol	1 - 500 ng/mL	TBD	Human plasma
Digoxin	0.1 - 20 ng/mL	200 µL	Human serum
Digoxin	5 - 1000 ng/mL	100 µL	Human urine
Available in July 2010 Docetaxel	TBD	TBD	Human plasma
Donepezil	0.2 - 100 ng/mL	200 µL	Human plasma
Duloxetine	0.2 - 200 ng/mL	50 µL	Human plasma
Erlotinib	1 - 3000 ng/mL	50 µL	Human plasma
5-Fluorouracil (5-FU)	2 - 1000 ng/mL; 10 - 10000 ng/mL	100 µL	Human plasma
Fenofibric Acid	20 - 10000 ng/mL	100 µL	Human plasma
Gemcitabine	10 - 10000 ng/mL	25 µL	Human plasma
Glyburide (non-protein bound)	0.025 - 2 ng/mL	100 µL	Human PFF
Glyburide (total)	1 - 250 ng/mL	200 µL	Human plasma
Imatinib (Gleevec®)/ N-Desmethyl Imatinib	100 - 10000 ng/mL 50 - 5000 ng/mL	50 µL	Human plasma
Lamivudine (as part of Combivir®)	2 - 1000 ng/mL	200 µL	Human plasma
Lamivudine (as part of Combivir®)	2 - 1000 ng/mL	200 µL	Human urine
Lovastatin/Lovastatin Acid	0.25 - 100 ng/mL	500 µL	Human plasma
Methotrexate/ 7-Hydroxymethotrexate	1 - 200 ng/mL 1 - 100 ng/mL	200 µL	Human plasma
Methotrexate/ 7-Hydroxymethotrexate	1000 - 50000 ng/mL 50 - 1000 ng/mL	20 µL	Human urine
Methylphenidate/α-Phenyl-α- (2-Piperidyl)Acetic Acid	0.05 - 25 ng/mL	100 µL	Human plasma
Midazolam/1-Hydroxymidazolam	0.1 - 100 ng/mL	100 µL	Human plasma
Moxifloxacin	25 - 5000 ng/mL	50 µL	Human plasma
Omeprazole/ 5-Hydroxyomeprazole; Omeprazole Sulfone	0.5 - 500 ng/mL 0.5 - 250 ng/mL	50 µL	Human plasma
Paclitaxel/6-Hydroxypaclitaxel	10 - 5000 ng/mL	200 µL	Human plasma
S,S-Reboxetine	0.5 - 500 ng/mL	50 µL	Human plasma
Ritonavir	10 - 5000 ng/mL	50 µL	Human plasma
Rosiglitazone	5 - 3000 ng/mL	25 µL	Human plasma
Rosuvastatin/ Rosuvastatin Lactone; N-Desmethyl Rosuvastatin	0.5 - 100 ng/mL 0.5 - 50 ng/mL	100 µL	Human plasma
Simvastatin/Simvastatin Acid	0.1 - 30 ng/mL	200 µL	Human plasma
Tolterodine/ 5-Hydroxymethyltolterodine	0.2 - 50 ng/mL	100 µL	Human plasma
Valproic Acid	1 - 100 µg/mL	100 µL	Human serum
R-Warfarin, S-Warfarin (chiral)	0.1 - 10 ng/mL	200 µL	Human PFF (non-protein bound)
R-Warfarin, S-Warfarin (chiral)	1 - 2500 ng/mL	100 µL	Human plasma (total)
Zidovudine (as part of Combivir®)	2 - 1000 ng/mL	200 µL	Human plasma
Zidovudine (as part of Combivir®)	2 - 1000 ng/mL	200 µL	Human urine
Ziprasidone	0.5 - 150 ng/mL	200 µL	Human plasma

Advion Offers Free Instructional Video

Advion offers an instructional video, “Techniques for the Packing and Shipment of Biological Samples”, to help ensure success of clinical studies. (more)

Advion Leader

Dr. Jack Henion
Co-founder, Chairman and Chief Scientific Officer

"My vision for Advion is to be the premier bioanalytical laboratory by always striving for the highest standards of scientific excellence, technology leadership, and unparalleled service."